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Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria
https://ir.soken.ac.jp/records/3872
https://ir.soken.ac.jp/records/3872896349b4-cdb9-4f24-8bb8-ad8137a2f677
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-12 | |||||
タイトル | ||||||
タイトル | Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
OKAJIMA, Koji
× OKAJIMA, Koji× YOSHIHARA, Shizue× FUKUSHIMA, Yoshimasa× GENG, Xiaoxing× KATAYAMA, Mitsunori× HIGASHI, Shoichi× WATANABE, Masakatsu× SATO, Shusei× TABATA, Satoshi× SHIBATA, Yutaka× ITOH, Shigeru× IKEUCHI, Masahiko |
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著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, TII0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared TII0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. TII0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the "short" BLUF proteins in the regulation of cyanobacterial phototaxis. | |||||
書誌情報 |
Journal of Biochemistry en : Journal of Biochemistry 巻 137, 号 6, p. 741-750, 発行日 2005-06 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021924X | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 16002996 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1093/jb/mvi089 | |||||
関連名称 | 10.1093/jb/mvi089 | |||||
権利 | ||||||
権利情報 | © 2005 The Japanese Biochemical Society |