WEKO3
アイテム
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It was originarry found in three mammalian\u003cbr /\u003e transcription factors, the pituitary-specific Pit-1/GHF-1,the\u003cbr /\u003e ubiquitous Oct-1 and the predominantly B cell specific Oct-2, and\u003cbr /\u003e the product of the cell lineage control gene \u003cu\u003eUnc\u003c/u\u003e-86 of \u003cu\u003eCaenorhadbitis\u003c/u\u003e\u003cbr /\u003e\u003cu\u003eelegans\u003c/u\u003e (Herr \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,1988 and references therein). \u003cbr /\u003eBy means of\u003cbr /\u003e sequence similarity, several other mammalian POU domain genes have\u003cbr /\u003e also been identified (He \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal.\u003c/u\u003e, 1989; Monouki \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal.\u003c/u\u003e, 1990; Okamoto\u003cbr /\u003e \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal.\u003c/u\u003e, 1990; Rosner et al., 1990 Scholer \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal.\u003c/u\u003e, 1990; Suzuki \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,\u003cbr /\u003e1990). All of them were shown to interact with an octamer-like\u003cbr /\u003e sequence and to activate transcription \u003cu\u003evia\u003c/u\u003e an octamer motif near the\u003cbr /\u003e TATA box.The \u003cu\u003eDrosophila\u003c/u\u003e Cfl-a protein,which interants with a DNA\u003cbr /\u003e element required for expression of the dopa decarboxylase gene in\u003cbr /\u003e selected dopaminergic neurons (Johnson and Hirsh,1990), was also\u003cbr /\u003e found to posses a POU domain similar to those of the mouse Oct-6\u003cbr /\u003e (Suzuki \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,1990) and the humann Brn-1 and Brn-2 (He \u003cu\u003eet\u003c/u\u003e al\u003c/u\u003e.,1898)\u003cbr /\u003eproteins. These POU domain genes are likely regulatory genes\u003cbr /\u003e controlling transcription of distinct sets of genes during develop-\u003cbr /\u003ement. The finding that two dwarf mutations in mice are null mutations\u003cbr /\u003e in the Pit-1/GHF-1 gene (Li \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990) provide further support on\u003cbr /\u003e the roles of POU transcription factors in development. Recently, the\u003cbr /\u003e mternally expressed POU transcription factor, the Oct-3/4\u003cbr /\u003e protein (Okamoto \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990; Scholer \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990), has also been\u003cbr /\u003e shown to be required for the first embryonic cell division in mice\u003cbr /\u003e (Rosner \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990).\u003cbr /\u003e Suzuki and his colleagues have been studying the developmental\u003cbr /\u003e regulation of the silk protein genes in \u003cu\u003eBonbyx\u003c/u\u003e \u003cu\u003emori\u003c/u\u003e\u003c/i\u003e (Suzuki \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,\u003cbr /\u003e 1990). Among them, the siricin-1 gene is expressed exclusively in the\u003cbr /\u003e middle silk gland while the fibroin gene is specific to the posterior\u003cbr /\u003e silk gland. Both genes are actively expressed during the intermolt\u003cbr /\u003e but repressed during the molting stages. Several silk gland protins\u003cbr /\u003e have been identified as putative regulatoly factors involved in the\u003cbr /\u003e transcriptioal control of the fibroin and scrin-1 genes ( Hui \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,\u003cbr /\u003e 1990; Matsuno \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1989,1990). One of these proeins , SGF-3, was\u003cbr /\u003e found to bind with high affinity to the SC region of the sericin-1 gene\u003cbr /\u003e and the distal upstream reion of the fibroin gene (Hui \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990).\u003cbr /\u003e These regions are known to be important for an efficient transcription\u003cbr /\u003e in the silk gland extracts. Amultimer of the SC region gave\u003cbr /\u003e transcriptional enhancement in extracts prepared from the middle silk\u003cbr /\u003e gland where the sericin-1 gene is specifically epressed but that of a\u003cbr /\u003e mutant SC region giving a reduced affinity for SGF-3 did not (Matsuno\u003cbr /\u003e \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990). Mobility shift assay revealed that SGF-3 is far more\u003cbr /\u003e abundant in the silk gland of the 2-day-old fifth-instar larvae than in\u003cbr /\u003e the pasterior silk gland (Hui \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990; Matsuno \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., 1990; Y.\u003cbr /\u003e Suzuki, unpublised). These obaervations suggest that the SGF-3 is a key\u003cbr /\u003e regulatory factor in the transcription control ol the sericin-1 gene. \u003cbr /\u003e The SGF-3 was supposed as an octamer binding protein (Hui \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e.,\u003cbr /\u003e 1990). Since high affnitiy SGF-3 binding sites, such as the SC and\u003cbr /\u003e fibroin distal upstream regions, also contain octamer-like sequences,\u003cbr /\u003e it has been speculated that SGF-3 might possess a POU domain similar\u003cbr /\u003e to the mammalian octamer-binding proteins. This repoort presents here\u003cbr /\u003e the isolation and characterization of a POU domain containg cDNA\u003cbr /\u003e (POU-M1) from the middle silk gland. It encodes a protein with a POU\u003cbr /\u003e domain identical to that of the \u003cu\u003eDrosophila\u003c/u\u003e \u003cu\u003eCfl-a\u003c/u\u003e protein. By mobility\u003cbr /\u003e shift assay and nuclease protection assay, the POU-M1 protein and the\u003cbr /\u003e putative silk gland factor SGF-3 were found to interact in an\u003cbr /\u003e indistinguishable manner with the SC region of the sericin-1 gene,\u003cbr /\u003e which is a key \u003cu\u003ecis\u003c/u\u003e-acting element involved in the stimulation of\u003cbr /\u003e sercin-1 gene transcription through the interaction with SGF-3.\u003cbr /\u003e Antibodies raised against the synthetic oligopeptides correponding\u003cbr /\u003e to the two regions of putative POU-M1 protein reacted specifically to\u003cbr /\u003e both the POU-M1 protein and SGF-3. Northern blot hybridization\u003cbr /\u003e and Western blotting revealed that the POU-M1 expression is regulated\u003cbr /\u003e both temporally and spatially during the silk gland development. It\u003cbr /\u003e is concluded that the POU-M1 proein is identical to the SGF-3 and\u003cbr /\u003e proposed that the differential expression of the POU-M1 gene is probably\u003cbr /\u003e involved in the transcriptional regulation of the silk protein genes.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第26号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "X2 分子生物機構論専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1991"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "(2018年2月16日)本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "FUKUTA, Masakazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9530", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲26_要旨.pdf", "filesize": [{"value": "275.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 275200.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/1296/files/甲26_要旨.pdf"}, "version_id": "e0b4ed7b-f333-4be9-a75a-52679072f32d"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲26_本文.pdf", "filesize": [{"value": "1.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1500000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1296/files/甲26_本文.pdf"}, "version_id": "d2c8dbcb-f4a7-43be-a536-3bc8c5df3abc"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "カイコセリシン-1遺伝子の制御に関わるPOUドメインを持つ転写因子のクローニング", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "カイコセリシン-1遺伝子の制御に関わるPOUドメインを持つ転写因子のクローニング"}, {"subitem_title": "Molecular cloning of a POU domain transcriptionfactor involved in regulation of Bombyxsericin-1 gene", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["27"], "permalink_uri": "https://ir.soken.ac.jp/records/1296", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1296", "relation": {}, "relation_version_is_last": true, "title": ["カイコセリシン-1遺伝子の制御に関わるPOUドメインを持つ転写因子のクローニング"], "weko_shared_id": 1}
カイコセリシン-1遺伝子の制御に関わるPOUドメインを持つ転写因子のクローニング
https://ir.soken.ac.jp/records/1296
https://ir.soken.ac.jp/records/12965f9ee1aa-c370-4b6d-b0fa-bfb674deb960
名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 / Abstract, Screening Result (275.2 kB)
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本文 (1.5 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2010-02-22 | |||||
タイトル | ||||||
タイトル | カイコセリシン-1遺伝子の制御に関わるPOUドメインを持つ転写因子のクローニング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Molecular cloning of a POU domain transcriptionfactor involved in regulation of Bombyxsericin-1 gene | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者名 |
福田, 雅一
× 福田, 雅一 |
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フリガナ |
フクタ, マサカズ
× フクタ, マサカズ |
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著者 |
FUKUTA, Masakazu
× FUKUTA, Masakazu |
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学位授与機関 | ||||||
学位授与機関名 | 総合研究大学院大学 | |||||
学位名 | ||||||
学位名 | 博士(理学) | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 総研大甲第26号 | |||||
研究科 | ||||||
値 | 生命科学研究科 | |||||
専攻 | ||||||
値 | X2 分子生物機構論専攻 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1992-03-16 | |||||
学位授与年度 | ||||||
1991 | ||||||
要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The POU domain is a DNA-binding regionconsisting of 75-82<br /> amino acids POU-specific domain, a short variable linker region and<br /> a POU-homeodomain of 60 amino acids (for a review,seeRuvkin and<br /> Finny, 1991). It was originarry found in three mammalian<br /> transcription factors, the pituitary-specific Pit-1/GHF-1,the<br /> ubiquitous Oct-1 and the predominantly B cell specific Oct-2, and<br /> the product of the cell lineage control gene <u>Unc</u>-86 of <u>Caenorhadbitis</u><br /><u>elegans</u> (Herr <u>et</u> <u>al</u>.,1988 and references therein). <br />By means of<br /> sequence similarity, several other mammalian POU domain genes have<br /> also been identified (He <u>et</u> <u>al.</u>, 1989; Monouki <u>et</u> <u>al.</u>, 1990; Okamoto<br /> <u>et</u> <u>al.</u>, 1990; Rosner et al., 1990 Scholer <u>et</u> <u>al.</u>, 1990; Suzuki <u>et</u> <u>al</u>.,<br />1990). All of them were shown to interact with an octamer-like<br /> sequence and to activate transcription <u>via</u> an octamer motif near the<br /> TATA box.The <u>Drosophila</u> Cfl-a protein,which interants with a DNA<br /> element required for expression of the dopa decarboxylase gene in<br /> selected dopaminergic neurons (Johnson and Hirsh,1990), was also<br /> found to posses a POU domain similar to those of the mouse Oct-6<br /> (Suzuki <u>et</u> <u>al</u>.,1990) and the humann Brn-1 and Brn-2 (He <u>et</u> al</u>.,1898)<br />proteins. These POU domain genes are likely regulatory genes<br /> controlling transcription of distinct sets of genes during develop-<br />ment. The finding that two dwarf mutations in mice are null mutations<br /> in the Pit-1/GHF-1 gene (Li <u>et</u> <u>al</u>., 1990) provide further support on<br /> the roles of POU transcription factors in development. Recently, the<br /> mternally expressed POU transcription factor, the Oct-3/4<br /> protein (Okamoto <u>et</u> <u>al</u>., 1990; Scholer <u>et</u> <u>al</u>., 1990), has also been<br /> shown to be required for the first embryonic cell division in mice<br /> (Rosner <u>et</u> <u>al</u>., 1990).<br /> Suzuki and his colleagues have been studying the developmental<br /> regulation of the silk protein genes in <u>Bonbyx</u> <u>mori</u></i> (Suzuki <u>et</u> <u>al</u>.,<br /> 1990). Among them, the siricin-1 gene is expressed exclusively in the<br /> middle silk gland while the fibroin gene is specific to the posterior<br /> silk gland. Both genes are actively expressed during the intermolt<br /> but repressed during the molting stages. Several silk gland protins<br /> have been identified as putative regulatoly factors involved in the<br /> transcriptioal control of the fibroin and scrin-1 genes ( Hui <u>et</u> <u>al</u>.,<br /> 1990; Matsuno <u>et</u> <u>al</u>., 1989,1990). One of these proeins , SGF-3, was<br /> found to bind with high affinity to the SC region of the sericin-1 gene<br /> and the distal upstream reion of the fibroin gene (Hui <u>et</u> <u>al</u>., 1990).<br /> These regions are known to be important for an efficient transcription<br /> in the silk gland extracts. Amultimer of the SC region gave<br /> transcriptional enhancement in extracts prepared from the middle silk<br /> gland where the sericin-1 gene is specifically epressed but that of a<br /> mutant SC region giving a reduced affinity for SGF-3 did not (Matsuno<br /> <u>et</u> <u>al</u>., 1990). Mobility shift assay revealed that SGF-3 is far more<br /> abundant in the silk gland of the 2-day-old fifth-instar larvae than in<br /> the pasterior silk gland (Hui <u>et</u> <u>al</u>., 1990; Matsuno <u>et</u> <u>al</u>., 1990; Y.<br /> Suzuki, unpublised). These obaervations suggest that the SGF-3 is a key<br /> regulatory factor in the transcription control ol the sericin-1 gene. <br /> The SGF-3 was supposed as an octamer binding protein (Hui <u>et</u> <u>al</u>.,<br /> 1990). Since high affnitiy SGF-3 binding sites, such as the SC and<br /> fibroin distal upstream regions, also contain octamer-like sequences,<br /> it has been speculated that SGF-3 might possess a POU domain similar<br /> to the mammalian octamer-binding proteins. This repoort presents here<br /> the isolation and characterization of a POU domain containg cDNA<br /> (POU-M1) from the middle silk gland. It encodes a protein with a POU<br /> domain identical to that of the <u>Drosophila</u> <u>Cfl-a</u> protein. By mobility<br /> shift assay and nuclease protection assay, the POU-M1 protein and the<br /> putative silk gland factor SGF-3 were found to interact in an<br /> indistinguishable manner with the SC region of the sericin-1 gene,<br /> which is a key <u>cis</u>-acting element involved in the stimulation of<br /> sercin-1 gene transcription through the interaction with SGF-3.<br /> Antibodies raised against the synthetic oligopeptides correponding<br /> to the two regions of putative POU-M1 protein reacted specifically to<br /> both the POU-M1 protein and SGF-3. Northern blot hybridization<br /> and Western blotting revealed that the POU-M1 expression is regulated<br /> both temporally and spatially during the silk gland development. It<br /> is concluded that the POU-M1 proein is identical to the SGF-3 and<br /> proposed that the differential expression of the POU-M1 gene is probably<br /> involved in the transcriptional regulation of the silk protein genes. | |||||
所蔵 | ||||||
値 | 有 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |