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Solution x-ray-scattering study on the chaperonin groel from escherichia-coli Solution x-ray-scattering study on the chaperonin groel from escherichia-coli 桑島, 邦博 IGARASHI, Yoshihiko KIMURA, Kazumoto ICHIMURA, Kaoru MATSUZAKI, Shigeru IKURA, Teikichi KUWAJIMA, Kunihiro KIHARA, Hiroshi metadata only access © 1995 Published by Elsevier B.V. The molecular architecture of native GroEL has been studied by solution X-ray scattering. The radius of gyration for the native molecule was estimated to be 66.0 Å in 50 mM Tris-HCl, 100 mM KCl at pH 7.5 and 25°C. The maximum dimension was estimated to be 170 Å, based on the pair distance distribution function. A cylindrical structure or two heptameric rings was found to be the best for native GroEL among structures examined by using a multi-sphere model analysis in which the radius of constituent sphere was 6 Å. The results of the model analysis show that the radius of GroEL is 68.0 Å and the height is 150.7 Å. Unexpectedly, the central penetrating hole through GroEL was not confirmed in the best-fit structure. Elsevier 1995-02 eng journal article https://ir.soken.ac.jp/records/4156 7880961 https://doi.org/10.1016/0301-4622(94)00107-U 10.1016/0301-4622(94)00107-U 0301-4622 Biophysical Chemistry Biophysical Chemistry 53 3 259 266