2026-03-10T18:42:28Z https://ir.soken.ac.jp/oai

oai:ir.soken.ac.jp:00004327 2023-06-20T14:24:58Z 1:323

The allosteric transition of the chaperonin groel fromescherichia coli as studied by solution X-ray scattering The allosteric transition of the chaperonin groel fromescherichia coli as studied by solution X-ray scattering 桑島, 邦博 KUWAJIMA, Kunihiro INOBE, Tomonao ARAI, Munehito metadata only access SpringerLink(The original publication is available at www.springerlink.com) This is a short review article of our recent studies on the ATP-induced, allosteric conformational transition of the chaperonin GroEL complex by solution X-ray scattering. We used synchrotron X-ray scattering with a twodimensional, charge-coupled, device-based X-ray detector to study (1) the specificity of the chaperonin GroEL for its ligand that induced the allosteric transition, and (2) the identification of the allosteric transition of GroEL in its complicated kinetics induced by ATP. Due to the dramatically increased sensitivity of the X-ray scattering technique based on the use of the two dimensional X-ray detector and synchrotron radiation, different allosteric conformational states of GroEL populated under different conditions were clearly distinguished from each other. It was concluded that solution X-ray scattering is an extremely powerful tool for investigating the equilibrium and kinetics of cooperative conformational transitions of oligomeric protein complex, especially when combined with other spectroscopic techniques such as fluorescence spectroscopy. SpringerLink 2006-04 eng journal article https://ir.soken.ac.jp/records/4327 https://doi.org/10.1007/BF03218504 10.1007/BF03218504 1598-5032 Macromolecular Research Macromolecular Research 14 2 166 172