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Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes 小林, 久人 コバヤシ, ヒサト KOBAYASHI, Hisato Imprinted genes in mammals show monoallelic expression dependent<br /> on parental origin and are often associated with differentially methylated regions<br /> (DMRs). There are two classes of DMR: germline DMRs acquire gamete-specific<br /> methylation in either spermatogenesis or oogenesis and maintain the allelic<br /> methylation differences throughout development; secondary DMRs establish<br /> differential methylation patterns after fertilization. Targeted disruption of some<br /> germline DMRs showed that they dictate the allelic expression of nearby<br /> imprinted genes and the establishment of the allelic methylation of secondary<br /> DMRs. However, how the imprinting machinery recognizes germline DMRs is<br /> unknown. As a step toward elucidating the sequence features of the germline<br /> DMRs, I have determined the extents and boundaries of 15 germline<br /> mouse DMRs (including 12 maternally methylated and 3 paternally methylated ones) in<br /> 12.5-dpc embryos and sperm by bisulfite sequencing. I found that the average<br /> size of the DMRs was 2.7 kb and that their average G+C content was 54.2%.<br /> Oligonucleotide content analysis of the DMR sequences revealed that, although<br /> they are generally CpG rich, the paternally methylated DMRs contain less CpGs<br /> than the maternally methylated ones. Furthermore, based on the SOM analysis,<br /> I found that most germline DMRs have features distinct from typical mouse<br /> sequences. My findings provide a basis for the further characterization of DMRs.<br /> application/pdf 総研大甲第957号 eng thesis https://ir.soken.ac.jp/records/1020 博士（理学） 2006-03-24 総合研究大学院大学 https://ir.soken.ac.jp/record/1020/files/甲957_要旨.pdf application/pdf 207.7 kB 2016-02-17 https://ir.soken.ac.jp/record/1020/files/甲957_本文.pdf application/pdf 1.6 MB 2016-02-17