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oai:ir.soken.ac.jp:00001350 2023-06-20T14:45:04Z 2:430:27

A study on tissue specificity of DBL-1 signaling pathway that regulates body length in Caenorhabditis elegans A study on tissue specificity of DBL-1 signaling pathway that regulates body length in Caenorhabditis elegans 吉田, 悟 ヨシダ, サトル YOSHIDA, Satoru 総合研究大学院大学 博士（理学） Size control is one of the fundamental subjects of biology. In nematode Caenorhabditis elegans, a TGF-β-like signaling pathway, Sma pathway, which is composed of the ligand DBL-1, serine/threonine protein kinase receptors SMA-6 and DAF-4, and cytoplasmic signaling components SMA- 2, SMA-3, and SMA-4, regulates body length of the worm. To further address the molecular mechanism of body length regulation in the nematode by the TGF-β-like signaling pathway, I examined the regional requirement for the type I receptor SMA-6. Using a SMA-6::GFP (green fluorescent protein) reporter gene, I found sma-6 to be highly expressed in the hypodermis in addition to pharynx and intestine, while the type II receptor DAF-4 is reported to be more broadly expressed. I then examined the ability of SMA-6 expressed in different regions of the C. elegans body to rescue the sma-6 phenotype (small) and found that hypodermal expression of SMA-6 is most important and sufficient for the growth and maintenance of body length. I also show that GATA sequences in the sma-6 promoter contribute to the hypodermal expression of sma-6. Finally, I show that the DBL-1 signaling in middle larval stage is significant to control body length. application/pdf 総研大甲第556号 thesis 2001-09-28 application/pdf application/pdf https://ir.soken.ac.jp/record/1350/files/甲556_要旨.pdf https://ir.soken.ac.jp/record/1350/files/甲556_本文.pdf https://ir.soken.ac.jp/records/1350 eng