{"created":"2023-06-20T13:20:57.784836+00:00","id":1032,"links":{},"metadata":{"_buckets":{"deposit":"1a2ba303-3151-48ea-9f9e-804ccce89448"},"_deposit":{"created_by":1,"id":"1032","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1032"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001032","sets":["2:430:20"]},"author_link":["0","0","0"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"兼崎, 琢麿"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"カネサキ, タクマ"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2007-03-23"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"Heterotrimeric G protein signaling is involved in various pathways in animal <br />development. During Drosophila gastrula ion, Ga12 (Cta)mediated signaling <br />controls the ventral cellular movements of epithelia. Here, I show that G Protein <br />signaling also regulates the organization of cortical actin cytoskeleton underlining <br />plasma membrane in the epithelial cells. Multiple Gα subfamilies, Gα12 and <br />Gαi, are involved in the actin organization, but a known ligand of Gα12, Fog, is <br />not required. When rie-8, which is required for plasma membrane-targeting of <br />whole G proteins and their signaling activity, is mutated, the cell surfaces become <br />unstable and show blebs, and entire cellular movements in gastrulation are <br />delayed. The mutants for the common cofactors of the Gα's, GB13F and Gγ1, <br />show the similar phenotypes. Ventral cells also showed blebs like the riｃ-8 <br />mutant when the cells are treated with inhibitors of actin polymerization. The <br />disruption of cortical actin in the mutants for G proteins is seen in all epithelial <br />cells, but the disorganization of the surface occurs only in the moving ventral cells. <br />This suggests that the organization of cortical actin seems to be required to <br />endure the stress, which the ventral cells receive when they move during <br />gastrulation. <br />   Besides, I also show evidence that heterotrimeric G protein signaling is <br />involved in the control of the actin dynamics in syncytial blastderm stage. In <br />this stage, nuclear division is carried out with the assistance of two architectures <br />of actin cytoskeleton; actin caps in interphase and pseudocleavage <br />furrow-assembled actin network in mitosis. G protein is colocalized with the caps <br />and network of actin throughout this stage. In the ric-8mutant, this localization <br />pattern is lost and actin cytoskeleton shows abnormal morphology. Using <br />live-imaging technique, it is revealed that the ric-8 mutant shows the delay of the <br />change of actin morphology. These results suggest that G protein regulates <br />timing of the dynamic change of actin during mitosis, and provide new insight <br />into the role of G protein signaling in early embryogenesis of Drosophi7a. <br />   In addition, here I introduce a novel mutant of ric-8 gene, ric8<sup>atx</sup>, in which <br />408th E is substituted to K. The ric8<sup>atx</sup> mutant embryo shows cta- or fog like <br />phenotype of gastrulation, which is milder than that of the ric-8 null mutant. <br />Consistently, the ric8<sup>atx</sup> mutation results in the Gα12-specific defect of targeting. <br />Although the role of Ric-8 has been inferred from the results of genetic analyses <br />and of some in vitro assays in past studies, the true molecular function of this <br />protein is stil1 unclear. The findings from the analyses of ric8<sup>atx</sup> promote <br />elucidating the mysterious function of Ric-8 protein in viva .","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第1061号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"18 遺伝学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2006"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KANESAKI, Takuma","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲1061_要旨.pdf","filesize":[{"value":"279.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/1032/files/甲1061_要旨.pdf"},"version_id":"5c11c9b1-b2a3-4170-a4dd-8657a15a5eff"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila"},{"subitem_title":"Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1032","relation_version_is_last":true,"title":["Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T16:09:40.226937+00:00"}