{"created":"2023-06-20T13:20:58.521598+00:00","id":1048,"links":{},"metadata":{"_buckets":{"deposit":"6690dc2b-e79f-4df0-935c-5357362ff7e0"},"_deposit":{"created_by":1,"id":"1048","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1048"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001048","sets":["2:430:20"]},"author_link":["0","0","0"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宮本, 貴史"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ミヤモト, タカシ"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2008-03-19"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"　　It is well known that transcription elongation and repair of UV－damage is coupled.<br />In eukaryotes，stalled RNA pol II recruits several repair proteins to from a complex<br />containing RNA pol II，TFIIH，and the repair proteins．In prokaryotes，stalled RNAP<br />recruits Mfd protein．Mfd has a domain homologous to the domain in UvrB which<br />recognizes a wide range of structurally diverse lesions in addition to a domain for<br />interaction with RNAP．Mfd binds to the DNA segment immediately upstream of the<br />stalled RNAP and pushes it towards downstream by using the energy of<br />ATP-hydrolysis to dissociate it from DNA，and speculated to recruit UvrA to perform<br />the repair similar to the global nuclear excision repair. These mechanisms provide the<br />concept on the triggering DNA repair rather than that on transcriptional regulation．<br />　　Although RNAP tends to stall at all kinds of UV-damages，the efficiency of stallat at <br />a UV-damage has not been measured．Therefore，it is possible that inhibition of<br />transcription is carried out by a mechanism other than the stall of RNAP．The stall<br />could be overcome by a read-through of the damaged base by misincorporation，<br />slippage，Jump，and switching the template strand．These considerations indicate the<br />mechanism of coulpling of UV-damages and initiation，because initiation generally has<br />the largest dynamic range of transcriptional regulation．Irrespective of the efforts to<br />find specific factors triggering the inhibition in initiation，such factors have not been<br />found yet, suggesting an alternative possibility that the triggering function has been<br />already installed in RNAP．<br />　　Transcription initiation is biochemically separated into several steps．RNAP in the<br />form of holoenzyme binds to a promoter and then DNA duplex in the complex is<br />partially unwound to expose the template strand of DNA by mostly unknown<br />mechanism. The resultant complex is called open complex and this complex was once<br />considered to be homogeneous．However，it is now known to involve two or more<br />conformations which have different catalytic properties，forming branched pathway<br />mechanism. One branch leads to produce the full-length transcripts（productive<br />pathway），While another leads to produce only short transcripts iteratively<br />synthesized by“moribund complex”which is defined as a complex that produces only<br />abortive but no full-length transcripts（non-productive pathway）.On some promoters，<br />moribund complex is converted into dead-end complex which still maintains transcript<br />but lacks elongation activity．Accumulation of dead-end complex cause less<br />stoichiometric synthesis of the full-length products in a single-round transcription<br />assay.<br />　　To examine the potential coupling and its mechanism，I have studied on the<br />relationship between transcription initiation and UV-damage by using purified<br />reconstitution system of E.coli．This well-characterized system allows me to consider<br />the relationship between the structure and the function．The system is composed of<br />RNAP holoenzyme，a promoter DNA，and four NTPs as substrates．I selected the T7A1<br />promoter because little moribund and dead-end complexes are accumulating on this<br />promoter，simplifying the analysis of the branched pathway. By irradiating the<br />promoter DNA with UV light，UV damages are generated at adjacent pyrimidine bases<br />in the promoter region. If the generated damages are up stream from the transcription<br />start site，such damages are nothing to do with elongation pause and thus their effect<br />on transcription must be limited to the effect on initiation．<br />　　In this study, UV-irradiation of the template DNA was found to enhance abortive<br />initiation and to induce dead-end complex，namely enhancing the nonproductive<br />pathway．The positions of UV-damage in the template DNA in moribund and dead-end<br />complex was compared with those in the binary complex that contains productive<br />complex．The pyrimidine dimers at several sites in the upstream from the<br />transcription start site，but not all，were enriched in the moribund and dead-end<br />complexes．indicating that these UV-damaged pyrimidine bases induce these<br />complexes. These positions of the damages are on both template and non-template<br />strands. The induction by the damaged pyrimidine residues are confirmed by the<br />transcription on DNA fragments which contain single UV-damageds in the upstream<br />region．Furthermore，I found that adjacent pyrimidine bases are conserved atone or<br />more positions of those identified on T7A1 promoter among the majority of the E．coli<br />promoters，suggesting that these positions would be UV-sensor motif in promoters．<br />","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第1166号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"18 遺伝学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2007"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIYAMOTO, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲1166_要旨.pdf","filesize":[{"value":"270.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/1048/files/甲1166_要旨.pdf"},"version_id":"dd385290-e2f5-4420-8df4-e7773081ebce"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"UV-sensor motifs in promoters","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"UV-sensor motifs in promoters"},{"subitem_title":"UV-sensor motifs in promoters","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1048","relation_version_is_last":true,"title":["UV-sensor motifs in promoters"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T16:09:51.574006+00:00"}