@misc{oai:ir.soken.ac.jp:00001054,
 author = {小林, 未佳 and コバヤシ, ミカ and KOBAYASHI, Mika},
 month = {2016-02-17, 2016-02-17},
 note = {Estrogens play a central role in the reproduction of vertebrates and affect a variety of biological processes. The major target molecules of estrogens are nuclear estrogen receptors (ERs), which have been studied extensively at the molecular level. However, it remains unclear which genes are regulated directly by ERs, especially at the level of the whole organism rather than cultured cells. To identify the genes that are regulated directly by ERs in vivo, I subjected estrogen-treated mouse uteri to a modified chromatin immunoprecipitation (ChIP)-mediated target cloning strategy (Chapter 1).<br />　　　Sequence analysis of one of the anti-ERα antibodyprecipitated DNA fragments and alignment with the mouse genomic sequence revealed that the promoter region of the gene encoding aquaporin 5 (AQP5) contains an estrogen response element (ERE)(Chapter 2). Quantitative polymerase chain reaction (PCR) and DNA microarray analyses confirmed that AQP5 is activated soon after the administration of estrogen. Reporter assays also revealed that the 4 ERE in the promoter region of AQP5 is functional and is activated directly by estrogen. In contrast, while AQP8 is also expressed in the estrogen-treated uterus, reporter assays failed to detect a functional ERE. None of the other AQP genes responded to estrogen. The ChIP-mediated target identification strategy employed in this study may be useful for the study of other transcription factor networks., 総研大甲第1006号},
 title = {Chromatin‐Immunoprecipitation‐Mediated Target Identification Proved Aquaporin 5 is Regulated Directly by Estrogen in the Mouse Uterus},
 year = {}
}