{"created":"2023-06-20T13:21:00.346975+00:00","id":1087,"links":{},"metadata":{"_buckets":{"deposit":"4fee4277-5199-4b4f-8f56-614ccec64d59"},"_deposit":{"created_by":1,"id":"1087","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1087"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001087","sets":["2:430:22"]},"author_link":["9281","9280","9279"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石原, 悟"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"イシハラ, サトル"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1997-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（学術）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"　　　β catenin is involved not only in the cadherin-based cell adhesion system but also in the Wnt signaling pathway. In this pathway, it is supposed that β catenin is phosphorylated by glycogen synthase kinase-3β(GSK-3β) to become susceptible to degradation, that the Wnt signaling suppresses GSK-3β to increase the amount of non-phosphorylated, stable β catenin, and that the stable β catenin affects the transcription of various genes in the nucleus. To separate the role of β catenin in intracellular signaling from its role in the cadherin-based cell adhesion, they constructed a mutant β catenin(mbH) with amino acid substitutions at its putative GSK-3β phosphorylation site (GSKP sequence), and introduced it into L fibroblasts that lack the cadherin expression. In stable transfectants (LmbH), not only mbH but also endogenous wild type β catenin was stabilized. Green fluorescence protein (GFP) carrying the mutated GSKP sequence also stabilized the endogenous β catenin in L cells, indicating that the mutated GSKP sequence suppresses the degradation machinery for β catenin. Furthermore, by adding the nuclear localization signal to mbH, they obtained L transfectants (LmbHN) expressing the stabilized β catenin predominantly in the nucleus. Under the long-term aggregation conditions, LmbHN formed compact cell aggregates consisting mainly of 2-5 cells. LmbH also showed a similar aggregation activity but to a lesser extent, and parent L cells mostly remained to be dissociated. Close analyses by immunofluorescence microscopy and the cell adhesion inhibition assay with RGD peptides revealed that aggregation activity is attributed to the integrin-based cell adhesion. Considering that the amount of stabilized β catenin in the nucleus is significantly larger in LmbHN than in LmbH, they speculate that the accumulation of β catenin in the nucleus resulted in the activation of integrin-based cell adhesion. The transfectants established in this study, thus, will provide an advantageous system to selectively analyze the role of β catenin in the nucleus and in the regulation of integrin activity. ","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第281号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1996"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ISHIHARA, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲281_要旨.pdf","filesize":[{"value":"181.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1087/files/甲281_要旨.pdf"},"version_id":"9d589575-e105-426b-90af-09720f802efc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲281_本文.pdf","filesize":[{"value":"4.0 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1087/files/甲281_本文.pdf"},"version_id":"b215290f-fa52-4221-88ac-7d7524b370e4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus"},{"subitem_title":"Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1087","relation_version_is_last":true,"title":["Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:48:19.228964+00:00"}