{"created":"2023-06-20T13:21:01.199744+00:00","id":1106,"links":{},"metadata":{"_buckets":{"deposit":"e84fedde-b3e2-4d68-aa2b-e67439f5cbf6"},"_deposit":{"created_by":1,"id":"1106","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1106"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001106","sets":["2:430:22"]},"author_link":["9338","9337","9336"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"奥村, 哲"}],"nameIdentifiers":[{"nameIdentifier":"9336","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"オクムラ, テツ"}],"nameIdentifiers":[{"nameIdentifier":"9337","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2000-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"Part 1:  Relative distribution of cholinergic neurons vs. serotonergic fibers in the cat neostriatum<br />  The distribution of choke acetyltransferase (ChAT)-containing neurons and serotonin (5-HT)-containing nerve fibers in the cat neostriatum was investigated by use of immunohistochemical techniques.  Both ChAT- and 5-HT-staining techniques were applied to alternate brain sections, thereby allowing a precise comparison of the distribution pattern of ChAT-immunopositive cells (ChAT cells) and 5-HT-immunopositive fibers (5-HT fibers).  In the neostriatum, ChAT cells were strongly stained throughout their cell bodies and proximal (1st-order) dendrites.  The majority of them were multipolar cells with a soma diameter of 20-50 μm (long axis) X 10-30 μm (shirt axis).  In the caudate nucleus, ChAT cells were evenly and diffusely distributed except for the dorsolateral region of its rostral half;  in which latter region they were distributed in loosely formed clusters.  In the rostral portion of the putamen, the density of ChAT-cell distribution was like that in the medial region of the caudate nucleus.  In contrast, this distribution was more dense in the caudomedial region of the putamen, adjacent to the globus pallidus.  5-HT fibers in the neostriatum were dark-stained, of quite fine diameter (< 0.6 μm), and they contained small, round varicosities (diameter, usually 0.5-1.0 μm, but some >1.0 μm).  Such 5-HT fibers were distributed abundantly throughout the caudate nucleus and putamen.  In the rostrocauda1 portion of the caudate nucleus, their density was high in its dorsal and ventral components, and low in the middle component.  Throughout the putamen, 5-HT fibers were distributed homogeneously in the mediolateral and dorsoventral directions.  In the caudal portion of the putamen adjacent to the globus pallidus, the 5-HT fibers had a higher density while maintaining their homogenous distribution pattern.  In the two main divisions of the striatum, the so-called \"patch\" (Ache-poor) and \"matrix\" (AchE-rich) compartments, there was a near-even distribution of 5-HT fibers and terminals.  The above results suggest that the 5-HT-dominated, raphe-striatal pathway is optimally arranged for modulating the activity of both the intrinsic and the projection neurons of the neostriatum.<br /><br />Part 2: Distribution and fine morphology of neostriatal output neurons, and serotonergic innervation to the output neurons and cholinergic interneurons in the cat<br />  To characterize the distribution and fine morphology of neostriatal output neurons, retrograde neural tracer, biotinylated dextran amine (BDA) was focally injected into three major target nuclei of the striatum:  the globus pallidus (GP), entopeduncular nucleus (EP) and substantia nigra pars reticulata (SNR).  We found a general tendency that GP- and EP-projection cells to occur at highest density in the dorsal part of the caudate body and caudate head, respectively, whereas SNR-projection cells had their highest density in a more ventral portion of the head and body of the caudate nucleus.  At those rostro-caudal levels where the density of GP-, EP- and SNR-projection cells were highest, the density of BDA retrogradely labeled cells in the putamen tended to be the highest.  The majority of the labeled output projection neurons in the caudate nucleus were small to medium sized and multipolar in shape. The range of their soma diameters was 12-27 μm (long axis) X 6-17 μm (short axis).  They had 4-8 primary (1st-order) dendrites.  A number of spines on the remote dendrites were also BDA-labeled.  At the primary dendrites and the initial part of the secondary dendrites, spines tended to be absent.  There was no significant morphological difference in the characteristics and the size of the retrogradely labeled output cells with BDA injections into the GP, EP and SNR. 5-HT- and ChAT-immunohistochemistry were also applied for the neostriatal sections to investigate 5-HT innervation to BDA-labeled output neurons and ChAT positive interneurons.  ChAT positive cells were large-sized and aspiny.  Sonata and dendrites of output neurons projecting to the GP, EP and SNR and those of ChAT positive interneurons were densely surrounded by thin 5-HT fibers (C-fibers) and varicosities.  In addition, the spines of striatal output neurons were closely apposed by a number of fine 5-HT varicosities.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第475号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1999"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKUMURA, Tetsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9338","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲475_要旨.pdf","filesize":[{"value":"331.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1106/files/甲475_要旨.pdf"},"version_id":"27aad28b-e206-431e-b815-c727fa070227"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲475_本文.pdf","filesize":[{"value":"3.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1106/files/甲475_本文.pdf"},"version_id":"e4e821ff-4e0e-43af-b17d-6d363518fafa"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"PATTERN OF SEROTONERGIC INNERVATION TO INTRINSIC AND OUTPUT NEURONS IN THE CAT NEOSTRIATUM","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PATTERN OF SEROTONERGIC INNERVATION TO INTRINSIC AND OUTPUT NEURONS IN THE CAT NEOSTRIATUM"},{"subitem_title":"PATTERN OF SEROTONERGIC INNERVATION TO INTRINSIC AND OUTPUT NEURONS IN THE CAT NEOSTRIATUM","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1106","relation_version_is_last":true,"title":["PATTERN OF SEROTONERGIC INNERVATION TO INTRINSIC AND OUTPUT NEURONS IN THE CAT NEOSTRIATUM"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:47:49.983440+00:00"}