{"created":"2023-06-20T13:21:01.375481+00:00","id":1110,"links":{},"metadata":{"_buckets":{"deposit":"6ecd5fb3-7272-4385-9313-ddfda832e408"},"_deposit":{"created_by":1,"id":"1110","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1110"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001110","sets":["2:430:22"]},"author_link":["9350","9349","9348"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"范, 海天"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ハン, カイテン"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2000-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"Phloretin, which is the aglucon of phloridzin, has been widely used as an inhibitor of glucose transporter. Since we have recently found that phloretin inhibits a regulatory volume decrease after osmotic swelling in a human epithelial cell line, in the present study, I investigated the effects of phloretin on volume-sensitive Cl- channels using three different epithelial cell lines by the whole-cell patch-clamp technique. Extracellular application of phloretin (over 10 μM) was found to inhibit, in a concentration-dependent manner (IC50～20 μM), the volume-sensitive Cl- current activated by a hypotonic challenge in human epithelial T84 and Intestine 407 cells as well as in mouse epithelial C127/CFTR cells. In contrast, at a low concentration (30 μM), phloretin failed to inhibit CFTR Cl- currents activated by cAMP stimulation in T84 and C127/CFTR cells. At a high concentration (300 μM), however, partial inhibition by phloretin was observed in the CFTR Cl- currents in both cell types. At 30 and 300 μM, on the other hand, phloretin showed no inhibitory effect on Ca2+- activated Cl- currents induced by ionomycin in human T84 cells but rather reactivated them after inactivation during stimulation with ionomycin. It is concluded that phloretin is a novel inhibitor of volume-sensitive Cl- channel, and that at low concentrations phloretin specifically blocks this channel without inhibiting both cAMP-activated and Ca2+activated Cl- channels in epithelial cells.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第479号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1999"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FAN,  Hai-Tian","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲479_要旨.pdf","filesize":[{"value":"190.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1110/files/甲479_要旨.pdf"},"version_id":"461b3219-826a-4ea9-90fb-5c16b8af1034"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲479_本文.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1110/files/甲479_本文.pdf"},"version_id":"f680c3d4-09ec-47c4-9f47-43640e2a310c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells"},{"subitem_title":"Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1110","relation_version_is_last":true,"title":["Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:47:45.923315+00:00"}