@misc{oai:ir.soken.ac.jp:00001172,
 author = {本蔵, 直樹 and ホンクラ, ナオキ and HONKURA, Naoki},
 month = {2016-02-17, 2016-02-17},
 note = {The major cytoskeleton of dendritic spines is filamentous actin (F-actin).<br />Organizations of F-actin within individual spines, however, have not been<br />elucidated.　I have here investigated sub-spine actin organizations using<br />two-photon photoactivation of PA-GFP fused with β-actin in rat CA1 pyramidal<br />neurons in slice culture preparations.　I found segregated and discontinuous<br /.>organizations of two pools of F-actin, dynamic and stable pools, which tuned<br />over with time constants of 1.2 min and 17 min, respectively.　The stable F-actin<br />pool was localized at the base of spine head, often intruding into thick spine neck,<br />while the dynamic pool occupied the rest of spine head.　Fractions of the stable<br />F-actin pool were greater in larger spines, therefore, the entire F-actin poor was<br />more stable in larger spines.　I succeeded in visualizing a retrograde flow of<br />F-actin in the dynamic pool from the apex to the base of spine, and found that<br />both the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow<br />were greater in spines with larger head volumes.　Moreover, spine heads<br />rapidly shrank when actin polymerization was blocked by latrunculin A,<br />suggesting that the rate of actin polymerization in each spine actively and<br />continuously determines the volume of spine head via the length of F-actin.<br />Thus, I have revealed the sub-spine organizations of actin filaments that play<br />key role in spine structures and diversity., application/pdf, 総研大甲第970号},
 title = {Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation},
 year = {}
}