{"created":"2023-06-20T13:21:04.639718+00:00","id":1172,"links":{},"metadata":{"_buckets":{"deposit":"148832ac-897b-4b0d-9f78-50011e3ee2f2"},"_deposit":{"created_by":1,"id":"1172","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1172"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001172","sets":["2:430:22"]},"author_link":["0","0","0"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本蔵, 直樹"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ホンクラ, ナオキ"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2006-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"The major cytoskeleton of dendritic spines is filamentous actin (F-actin).
Organizations of F-actin within individual spines, however, have not been
elucidated. I have here investigated sub-spine actin organizations using
two-photon photoactivation of PA-GFP fused with β-actin in rat CA1 pyramidal
neurons in slice culture preparations. I found segregated and discontinuous
organizations of two pools of F-actin, dynamic and stable pools, which tuned
over with time constants of 1.2 min and 17 min, respectively. The stable F-actin
pool was localized at the base of spine head, often intruding into thick spine neck,
while the dynamic pool occupied the rest of spine head. Fractions of the stable
F-actin pool were greater in larger spines, therefore, the entire F-actin poor was
more stable in larger spines. I succeeded in visualizing a retrograde flow of
F-actin in the dynamic pool from the apex to the base of spine, and found that
both the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow
were greater in spines with larger head volumes. Moreover, spine heads
rapidly shrank when actin polymerization was blocked by latrunculin A,
suggesting that the rate of actin polymerization in each spine actively and
continuously determines the volume of spine head via the length of F-actin.
Thus, I have revealed the sub-spine organizations of actin filaments that play
key role in spine structures and diversity.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第970号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2005"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HONKURA, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"0","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲970_要旨.pdf","filesize":[{"value":"325.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/1172/files/甲970_要旨.pdf"},"version_id":"c0ec0d2f-b1ab-4a1c-85f7-46d3d3731b9d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲970_本文.pdf","filesize":[{"value":"6.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1172/files/甲970_本文.pdf"},"version_id":"28666919-3b8d-458b-87fc-d2410b595c80"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"},{"subitem_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1172","relation_version_is_last":true,"title":["Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T16:07:55.740482+00:00"}