{"created":"2023-06-20T13:21:04.639718+00:00","id":1172,"links":{},"metadata":{"_buckets":{"deposit":"148832ac-897b-4b0d-9f78-50011e3ee2f2"},"_deposit":{"created_by":1,"id":"1172","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1172"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001172","sets":["2:430:22"]},"author_link":["0","0","0"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本蔵, 直樹"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ホンクラ, ナオキ"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2006-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"The major cytoskeleton of dendritic spines is filamentous actin (F-actin).<br />Organizations of F-actin within individual spines, however, have not been<br />elucidated.　I have here investigated sub-spine actin organizations using<br />two-photon photoactivation of PA-GFP fused with β-actin in rat CA1 pyramidal<br />neurons in slice culture preparations.　I found segregated and discontinuous<br /.>organizations of two pools of F-actin, dynamic and stable pools, which tuned<br />over with time constants of 1.2 min and 17 min, respectively.　The stable F-actin<br />pool was localized at the base of spine head, often intruding into thick spine neck,<br />while the dynamic pool occupied the rest of spine head.　Fractions of the stable<br />F-actin pool were greater in larger spines, therefore, the entire F-actin poor was<br />more stable in larger spines.　I succeeded in visualizing a retrograde flow of<br />F-actin in the dynamic pool from the apex to the base of spine, and found that<br />both the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow<br />were greater in spines with larger head volumes.　Moreover, spine heads<br />rapidly shrank when actin polymerization was blocked by latrunculin A,<br />suggesting that the rate of actin polymerization in each spine actively and<br />continuously determines the volume of spine head via the length of F-actin.<br />Thus, I have revealed the sub-spine organizations of actin filaments that play<br />key role in spine structures and diversity.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第970号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2005"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HONKURA, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲970_要旨.pdf","filesize":[{"value":"325.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/1172/files/甲970_要旨.pdf"},"version_id":"c0ec0d2f-b1ab-4a1c-85f7-46d3d3731b9d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲970_本文.pdf","filesize":[{"value":"6.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1172/files/甲970_本文.pdf"},"version_id":"28666919-3b8d-458b-87fc-d2410b595c80"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"},{"subitem_title":"Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1172","relation_version_is_last":true,"title":["Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-06-20T16:07:55.740482+00:00"}