{"created":"2023-06-20T13:21:05.248007+00:00","id":1181,"links":{},"metadata":{"_buckets":{"deposit":"4b44ed9d-ab12-493f-8853-4b862a1a2432"},"_deposit":{"created_by":1,"id":"1181","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1181"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001181","sets":["2:430:22"]},"author_link":["0","0","0"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"児玉, 貴史"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"コダマ, タカシ"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2006-09-29"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"The rocker mice are hereditary ataxic mutants, which carry a point mutation in the gene<br />encoding the Ca<sub>V</sub> 2.1 (P/Q-type) Ca<sup>2+</sup> channel α1 subunit. They show the mildest<br />phenotypes among the reported Ca<sub>V</sub> 2.1 mutant mice, allowing us to evaluate primary<br />symptoms caused by the Ca<sub>V</sub> 2.1 dysfunction. Although the basic properties of the<br />rocker mutant channel have been reported, their influence on physiological functions<br />remains unclear. Here, I studied the impacts of the rocker mutant Ca<sup>2+</sup> channel on<br />synaptic transmission especially in cerebellar Purkinje cells, where Ca<sub>V</sub> 2.1 Ca<sup>2+</sup> channel<br />mediates a major activity-dependent Ca<sup>2+</sup> influx. Despite of the small changes in<br />channel function, synaptic transmission in the parallel fiber (PF)-Purkinje cell (PC)<br />synapses was severely impaired, whereas the climbing fiber inputs onto PCs appeared to function normally. I further explored the origin of the transmission impairment in the<br />PF-PC synapses in rocker mice. Presynaptic functions of the PF-PC synapses, however,<br />were unexpectedly normal in terms of paired-pulse facilitation, sensitivity to<br />extracellular Ca<sup>2+</sup> concentration, and glutamate concentration in synaptic clefts.<br />Electron microscopic analyses including freeze-fracture replica labeling revealed that<br />both  the  number  and  density  of  postsynaptic  AMPA<br />(α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors substantially<br />decreased without gross structural changes of the PF-PC synapses. I also observed an<br />abnormal arborization of PC dendrites in young adult rocker mice (「1 month of age).<br />These lines of evidence suggest that the rocker mutation exerts its influence especially<br />on postsynaptic structures and functions in the PF-PC synapses.","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第1010号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"20 生理科学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2006"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KODAMA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲1010_要旨.pdf","filesize":[{"value":"195.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/1181/files/甲1010_要旨.pdf"},"version_id":"380c5751-46d9-4be5-a4d3-ef5319961443"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲1010_本文.pdf","filesize":[{"value":"19.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1181/files/甲1010_本文.pdf"},"version_id":"3fba4a9d-f761-4b47-8a54-329b7febf8b7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker"},{"subitem_title":"Physiological and morphological studies on cerebellar synapses of CaV2.2 mutant mice rocker","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["22"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1181","relation_version_is_last":true,"title":["Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T15:55:36.804606+00:00"}