{"created":"2023-06-20T13:21:10.907875+00:00","id":1296,"links":{},"metadata":{"_buckets":{"deposit":"aea58039-448f-4a0f-a277-69450d75c944"},"_deposit":{"created_by":1,"id":"1296","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1296"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001296","sets":["2:430:27"]},"author_link":["9528","9529","9530"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福田, 雅一"}],"nameIdentifiers":[{"nameIdentifier":"9528","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"フクタ, マサカズ"}],"nameIdentifiers":[{"nameIdentifier":"9529","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1992-03-16"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"　　　The POU domain is a DNA-binding regionconsisting of 75-82<br /> amino acids POU-specific domain, a short variable linker region and<br /> a POU-homeodomain of 60 amino acids (for a review,seeRuvkin and<br /> Finny, 1991). It was originarry found in three mammalian<br /> transcription factors, the pituitary-specific Pit-1/GHF-1,the<br /> ubiquitous Oct-1 and the predominantly B cell specific Oct-2, and<br /> the product of the cell lineage control gene <u>Unc</u>-86 of <u>Caenorhadbitis</u><br /><u>elegans</u> (Herr <u>et</u> <u>al</u>.,1988 and references therein). <br />By means of<br /> sequence similarity, several other mammalian POU domain genes have<br /> also been identified (He <u>et</u> <u>al.</u>, 1989; Monouki <u>et</u> <u>al.</u>, 1990; Okamoto<br /> <u>et</u> <u>al.</u>, 1990; Rosner et al., 1990 Scholer <u>et</u> <u>al.</u>, 1990; Suzuki <u>et</u> <u>al</u>.,<br />1990). All of them were shown to interact with an octamer-like<br /> sequence and to activate transcription <u>via</u> an octamer motif near the<br /> TATA box.The <u>Drosophila</u> Cfl-a protein,which interants with a DNA<br /> element required for expression of the dopa decarboxylase gene in<br /> selected dopaminergic neurons (Johnson and Hirsh,1990), was also<br /> found to posses a POU domain similar to those of the mouse Oct-6<br /> (Suzuki <u>et</u> <u>al</u>.,1990) and the humann Brn-1 and Brn-2 (He <u>et</u> al</u>.,1898)<br />proteins. These POU domain genes are likely regulatory genes<br /> controlling transcription of distinct sets of genes during develop-<br />ment. The finding that two dwarf mutations in mice are null mutations<br /> in the Pit-1/GHF-1 gene (Li <u>et</u> <u>al</u>., 1990) provide further support on<br /> the roles of POU transcription factors in development. Recently, the<br /> mternally expressed POU transcription factor, the Oct-3/4<br /> protein (Okamoto <u>et</u> <u>al</u>., 1990; Scholer <u>et</u> <u>al</u>., 1990), has also been<br /> shown to be required for the first embryonic cell division in mice<br /> (Rosner <u>et</u> <u>al</u>., 1990).<br />　　　Suzuki and his colleagues have been studying the developmental<br /> regulation of the silk protein genes in <u>Bonbyx</u> <u>mori</u></i> (Suzuki <u>et</u> <u>al</u>.,<br /> 1990). Among them, the siricin-1 gene is expressed exclusively in the<br /> middle silk gland while the fibroin gene is specific to the posterior<br /> silk gland. Both genes are actively expressed during the intermolt<br /> but repressed during the molting stages. Several silk gland protins<br /> have been identified as putative regulatoly factors involved in the<br /> transcriptioal control of the fibroin and scrin-1 genes ( Hui <u>et</u> <u>al</u>.,<br /> 1990;  Matsuno <u>et</u> <u>al</u>., 1989,1990). One of these proeins , SGF-3, was<br /> found to bind with high affinity to the SC region of the sericin-1 gene<br /> and the distal upstream reion of the fibroin gene (Hui <u>et</u> <u>al</u>., 1990).<br /> These regions are known to be important for an efficient transcription<br /> in the silk gland extracts. Amultimer of the SC region gave<br /> transcriptional enhancement in extracts prepared from the middle silk<br /> gland where the sericin-1 gene is specifically epressed but that of a<br /> mutant SC region giving a reduced affinity for SGF-3 did not (Matsuno<br /> <u>et</u> <u>al</u>., 1990). Mobility shift assay revealed  that SGF-3 is far more<br /> abundant in the silk gland of the 2-day-old fifth-instar larvae than in<br /> the  pasterior silk gland (Hui <u>et</u> <u>al</u>., 1990; Matsuno <u>et</u> <u>al</u>., 1990; Y.<br /> Suzuki, unpublised). These obaervations suggest that the SGF-3 is a key<br /> regulatory factor in the transcription control ol the sericin-1 gene. <br />　　　The SGF-3 was supposed  as an octamer binding protein (Hui <u>et</u> <u>al</u>.,<br /> 1990). Since high affnitiy SGF-3 binding sites, such as the SC and<br /> fibroin distal upstream regions, also contain octamer-like sequences,<br /> it has been speculated that SGF-3 might possess a POU domain similar<br /> to the mammalian octamer-binding proteins. This repoort presents here<br /> the isolation and characterization of a POU domain containg cDNA<br /> (POU-M1) from the middle silk gland. It encodes a protein with a POU<br /> domain identical to that of the <u>Drosophila</u> <u>Cfl-a</u> protein. By mobility<br /> shift assay and nuclease protection assay, the POU-M1 protein and the<br /> putative silk gland factor SGF-3 were found to interact in an<br /> indistinguishable manner with the SC region of the sericin-1 gene,<br /> which is a key <u>cis</u>-acting element involved in the stimulation of<br /> sercin-1 gene transcription through the interaction with SGF-3.<br /> Antibodies raised against the synthetic oligopeptides correponding<br /> to the two regions of putative POU-M1 protein reacted specifically to<br /> both the POU-M1 protein and SGF-3. Northern blot hybridization<br /> and Western blotting revealed that the POU-M1 expression is regulated<br /> both temporally and spatially during the silk gland development. It<br /> is concluded that the POU-M1 proein is identical to the SGF-3 and<br /> proposed that the differential expression of the POU-M1 gene is probably<br /> involved in the transcriptional regulation of the silk protein genes.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第26号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"X2 分子生物機構論専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1991"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUKUTA, Masakazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9530","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲26_要旨.pdf","filesize":[{"value":"275.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1296/files/甲26_要旨.pdf"},"version_id":"e0b4ed7b-f333-4be9-a75a-52679072f32d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲26_本文.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1296/files/甲26_本文.pdf"},"version_id":"d2c8dbcb-f4a7-43be-a536-3bc8c5df3abc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"カイコセリシン-１遺伝子の制御に関わるＰＯＵドメインを持つ転写因子のクローニング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"カイコセリシン-１遺伝子の制御に関わるＰＯＵドメインを持つ転写因子のクローニング"},{"subitem_title":"Molecular cloning of a POU domain transcriptionfactor involved in regulation of Bombyxsericin-1 gene","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["27"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1296","relation_version_is_last":true,"title":["カイコセリシン-１遺伝子の制御に関わるＰＯＵドメインを持つ転写因子のクローニング"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:46:21.296382+00:00"}