{"created":"2023-06-20T13:21:13.061983+00:00","id":1338,"links":{},"metadata":{"_buckets":{"deposit":"38ce16a7-769d-45c1-bae0-8a4936179d95"},"_deposit":{"created_by":1,"id":"1338","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1338"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001338","sets":["2:430:27"]},"author_link":["9497","9495","9496"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 清和"}],"nameIdentifiers":[{"nameIdentifier":"9495","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"モリタ, キヨカズ"}],"nameIdentifiers":[{"nameIdentifier":"9496","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1999-09-30"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"  He has identified a new member of the TGF-β superfamily, CET-1, from Caenorhabditis elegans.  cet-1::GFP fusion is expressed in the ventral nerve cord and other neurons.  He isolated cet-1 null mutant by Tc1 deletion method.  cet-1 nullmutants have shortened bodies and male tail abnormal phenotype resembling sma mutants.  These results suggest that cet-1, sma-2, sma-3, and sma-4 share a common  pathway.  Overexpression experiments demonstrated that cet-1 function requires wild type sma genes.  Interestingly, CET-1 appears to affect body length in a dose dependent manner.  Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild type worms elongated body length close to Jon mutants.  Furthermore, his results show that cet-1 controls body length not proportionally but in two particular regions of the worm probably by changing cell volume.  Moreover, genetic interaction of the cet-1 with mutants of the other TGF- β pathway in C. elegans, daf demonstrated that cet-1 is also involved at least partly in the daf signaling pathway.  In male sensory ray patterning, lack of cet-1 function results in ray fusions.  Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail.  His results show that cet-1 controls diversed biological processes during C. elegans development probably through different target genes.<br />  He also has identified two genes, CeBRAM-1A and CeBRAM-2B, which are similar to human BRAM-1 previously identified as a BMP receptor associated molecule, in C. elegans.  The C. elegans BRAMs (CeBRAM-1A and CeBRAM-2B) show significant amino acid identity with human BRAM-1, particularly in the C-terminal region.   CeBRAM-1A was found to associate with DAF-1, the type I receptor in the daf pathway in C elegans as well as a vertebrate BMP type I receptor BMPRIA.  CeBRAM-1A::GFP fusion protein is expressed mainly in amphid neurons such as ASK, ASH, and ASG etc, where DAF-1 is expressed. Loss-of-function of CeBRAM-1A gene showed that the mutant worms looked normal except that they showed a behavior of head-lifting phenotype.  Genetic interaction of CeBRAM-1A with previously known daf-7 TGF-β pathway mutants revealed that CeBRAM-1A negatively regulates the daf-7 TGF-β pathway and functions between daf-1 type I receptor and daf-14 SMAD.  On the other hand, CeBRAM-2B::GFP fusion protein was expressed strongly in pharyngeal muscle and intestinal cells. This expression pattern of CeBRAM-2B is similar to that of sma-6 type I receptor of the cet-1/sma pathway. Interestingly, double strand RNA interference (dsRNAi) of CeBR,AM-2B showed Lon phenotype.  These results strongly suggest that CeBRAM-1A and CeBRAM-2B may function as negative regulators of two distinct TGF-β signal pathways, the daf pathway and the sma pathway respectively in C. elegans.  Thus, he proposes that CeBRAM-1A and CeBRAM-2B define a novel class of molecules that serve as a negative regulator of TGF- f3 pathways acting downstream of the type I receptor.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大乙第71号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"X2 分子生物機構論専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"1999"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MORITA, Kiyokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9497","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"乙71_要旨.pdf","filesize":[{"value":"268.2 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1338/files/乙71_要旨.pdf"},"version_id":"a003eec5-eeba-4cef-b590-f7ddd75012e0"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"乙71_本文.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1338/files/乙71_本文.pdf"},"version_id":"48a2b65b-c542-4d0d-86c7-fbf58f4ca80f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans"},{"subitem_title":"The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["27"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1338","relation_version_is_last":true,"title":["The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:46:36.327936+00:00"}