{"created":"2023-06-20T13:21:13.723307+00:00","id":1348,"links":{},"metadata":{"_buckets":{"deposit":"b53a7797-c345-456a-855c-ef3c0bb68a77"},"_deposit":{"created_by":1,"id":"1348","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1348"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00001348","sets":["2:430:27"]},"author_link":["9665","9664","9663"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山口, 利男"}],"nameIdentifiers":[{"nameIdentifier":"9663","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ヤマグチ, トシオ"}],"nameIdentifiers":[{"nameIdentifier":"9664","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2001-03-23"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"The coloration of blue flowers depends on the production of the appropriate anthocyanin pigments, the presence of metal ions and co-pigments, and vacuolar pH. An increase in vacuolar pH enhances blue coloration, however, little is known about the proteins that are responsible for raising the vacuolar pH. The Japanese morning glory (Ipomoea nil) normally displays bright blue flower. One of the mutable allele, named purple-mutable (pr-m) confers purple flowers with blue sectors owing to recurrent somatic mutation from recessive pr-m allele to the blue Pr-r (Purple- revertant) allele. In this study, I described detail of the identification and characterization of the Pr gene, that is necessary for displaying blue flower in the I. nil, and discussed the observations with regard to the blue flower coloration. Germinal revertant carrying Pr-r allele homozygously that displays blue flower was successfully obtained from pr-m line, and compared with pr-m line. No alterations were found in the anthocyanin pigment compositions between the pr-m and Pr-r lines whereas the vacuolar pH in the pr-m was significantly lower than that in Pr-r. These results indicate that the function of the Pr gene is to raise vacuolar pH. By comparing the pr-m and Pr- r with simplified transposon display methods (STDM), the Pr gene also cDNA were successfully isolated. A Tpnl-related transposon, Tpn4 insertion was found within the exon 1 of the Pr gene in pr-m plant. This gene contained an open reading frame that is similar to the vacuolar Na+/H+ exchanger found in yeast, Arabidopsis, and rice. The Pr gene cDNA was able to complement the nhxl mutation for vacuolar Na+/H+ exchanger in yeast (Saccharomyces cerevisiae). A second copy for Pr gene was found in I. nil genome, but this copy was found to be pseudogene. The Pr gene was the most abundantly expressed in flower petals, and extensively found at around 12 h before flower opening, whereas no such increased expression was observed in the flower of Petunia hybrida. This result indicates the abundant expression of NHX1 genes before flower blooming was not common in all flowering plants. Since the NHX1 genes that promote the transport of Na+ into the vacuoles has been known to be involved in salt tolerance, we can add another important role for blue flower coloration in the Japanese morning glory by the vacuolar alkalization.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第539号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"X2 分子生物機構論専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2000"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMAGUCHI, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9665","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲539_要旨.pdf","filesize":[{"value":"203.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/1348/files/甲539_要旨.pdf"},"version_id":"c7efb042-3efd-4d62-a3d6-db08f9b290ff"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲539_本文.pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/1348/files/甲539_本文.pdf"},"version_id":"5fc99f8e-d46b-4461-b92b-8985476128e2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory"},{"subitem_title":"Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["27"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"1348","relation_version_is_last":true,"title":["Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:45:07.220274+00:00"}