@misc{oai:ir.soken.ac.jp:00001382,
 author = {鈴木, 真吾 and スズキ, シンゴ and SUZUKI, Shingo},
 month = {2016-02-17},
 note = {Phosphate is an essential nutrient and it is required, for example, for the biosynthesis of nucleotides, DNA and RNA, and for the functional regulation of proteins by phosphorylation. However, phosphate is one of the least available nutrients in the environment. Therefore, regulatory mechanisms have developed for the acquisition, storage, and metabolism of phosphate. One such regulatory mechanisms involves the induction of the expression of genes whose products maintain an appropriate range of phosphate concentrations within each cell under phosphate-limiting conditions. The primary event under such conditions is the perception of phosphate limiting conditions and transduction of the signal. However, the molecular mechanism by which organisms perceive the phosphate?limitation signal is still unclear.<br />　　　They identified previously a two component system, which consists of histidine kinase SphS and its cognate response regulator SphR, that regulates the expression of the phoA gene (sll0654) for alkaline phosphatase under phosphate-limiting conditions in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, he used DNA microarrays to investigate the role of SphS and SphR in the regulation of the genome-wide expression of genes in response to phosphate limitation. In wild-type cells, phosphate limitation induced the expression of 27 genes with induction factors greater than 2.5. Nineteen of these genes were included in five clusters of genes, namely, the pstl and pst2 clusters that encode phosphate transporters; the  phoA and nucH genes; genes for signal transducers; the sll0721 and sll0722 genes that encode proteins of  unknown function. Phosphate limitation repressed the expression of 18 genes with induction factors below 0.4.  These genes included the urtA gene and genes for ribosome proteins, in two clusters. Inactivation of either of  SphS or SphR completely eliminated the phosphate limitation inducible expression of genes and the phosphate  limitation repressible expression of the urtA gene. In addition, he found that SphR bound to the upstream  flanking regions of several phosphate limitation inducible genes at repetitive PyTTAAPyPy(T/A)-like sequences.  These results suggest that the SphS SphR two component system in Synechocystis sp. PCC 6803 is the  dominant sensory system that controls gene expression in response to phosphate limitation., 総研大甲第775号},
 title = {The SphS-SphR two-component system is the exclusive sensor for the induction of gene expression in response to phosphate limitation in Synechocystis sp.  PCC 6803;  A study of genome-wide expression of genes by DNA microarray analysis},
 year = {}
}