@article{oai:ir.soken.ac.jp:00003332, author = {渡辺, 正勝 and NAKAMURA, Shogo and MIKAMORI, Masahiro and HIRAMATSU, Mitsuo and EURA, Shigeru and TAKAMOTO, Hisayoshi and WATANABE, Masakatsu}, issue = {6}, journal = {Zoological Science, Zoological Science}, month = {Aug}, note = {application/pdf, Strong fluorescence emission occurred in sea urchin larvae when irradiated with blue light under a fluorescence microscope. The blue light irradiation first broke red granules in the pigment cells, releasing green fluorescent substance(s) into the cytoplasm of the pigment cells. The released and dispersed fluorescent substance(s) then made the entire pigment cell emit strong green fluorescence. With prolonged blue light irradiation, the pigment cell itself bursted, dispersing the fluorescent substance(s) into the body cavity or seawater. This resulted in "explosive emission" of the fluorescence. Cells that can emit such fluorescence first appeared at the late-blastula stage, proliferated with development, and changed into the red-pigmented cells. Similar fluorescence emission was observed in the larvae of Clypeaster japonicus, Hemicentrotus pulcherrimus, Anthocidaris crassispina and Pseudocentrotus depressus, of which C. japonicus larvae displayed the strongest fluorescence.}, pages = {807--810}, title = {Spectacular fluorescence emission in sea urchin larvae}, volume = {18}, year = {2001} }