@article{oai:ir.soken.ac.jp:00003706,
 author = {尚之, 高畑 and KONDO, Rumi and SATTA, Yoko and MATSUURA, Etsuko T and ISHIKAWA, H and TAKAHATA, Naoyuki and CHIGUSA, Sadao I},
 issue = {3},
 journal = {Genetics, Genetics},
 month = {},
 note = {The possibility of incomplete maternal transmission of mitochondrial DNA (mtDNA) in Drosophila, previously suggested by the presence of heteroplasmy, was examined by intra- and interspecific backcrosses of Drosophila simulans and its closest relative, Drosophila mauritiana. mtDNAs of offspring in these crosses were characterized by Southern hybridization with two alpha-32P-labeled probes that are specific to paternal mtDNAs. This method could detect as little as 0.03% paternal mtDNA, if present, in a sample. Among 331 lines that had been backcrossed for ten generations, four lines from the interspecific cross D. simulans (female) x D. mauritiana (male) showed clear evidence for paternal leakage of mtDNA. In three of these the maternal type was completely replaced while the fourth was heteroplasmic. Since in this experiment the total number of fertilization is known to be 331 x 10 = 3310, the proportion of paternal mtDNA per fertilization was estimated as about 0.1%. The mechanisms and evolutionary significance for paternal leakage are discussed in light of this finding.},
 pages = {657--663},
 title = {Incomplete maternal transmission of mitochondrial DNA in Drosophila},
 volume = {126},
 year = {1990}
}