{"created":"2023-06-20T13:23:05.827153+00:00","id":3862,"links":{},"metadata":{"_buckets":{"deposit":"6fca07f5-b3d5-4f8a-8f69-ad88793e4c00"},"_deposit":{"created_by":21,"id":"3862","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3862"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003862","sets":["1:283"]},"author_link":["345","1835","1836","1839","1838","1837","346","1834"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-08-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"1279","bibliographicPageStart":"1273","bibliographicVolumeNumber":"178","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications "},{"bibliographic_title":"Biochemical and Biophysical Research Communications ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 正勝"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A real-time automated method was developed for simultaneous measurements of phototactic orientation (phototaxis) and step-up photophobic response of flagellated microorganisms. Addition of all-trans retinal restored both photoresponses in a carotenoid-deficient mutant strain of Chlamydomonas reinhardtii in a dose-dependent manner. The phototactic orientation was biphasic with respect to both the light intensity and the concentration of retinal. All-trans retinal was more effective than 11-cis retinal to regenerate both photobehavioral responses. Analogs having locked 11-cis configurations and a phenyl ring in the side chain also induced photoresponses, although at concentrations more than two orders of magnitude higher than all-trans retinal. According to the present assay method, the responses were hardly detectable in cells incubated with retinal analogs in which the 13-ene was locked in either its trans or cis configuration. The results strongly suggest that the isomerization of the 13-14 double bond is important for photobehavioral signal transduction and that a single retinal-dependent photoreceptor controls both phototactic and photophobic responses.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"1872847","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0006-291X(91)91031-7 "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0006-291X(91)91031-7","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1991 Published by Elsevier Inc."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X ","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TAKAHASHI, Tetsuo "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHIHARA, Kazuo "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Masakatsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUBOTA, Mamoru "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"JOHNSON, Randy "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"DERGUINI, Fadila "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKANISHI, Koji "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses"},{"subitem_title":"Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["283"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-09"},"publish_date":"2013-08-09","publish_status":"0","recid":"3862","relation_version_is_last":true,"title":["Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:29:28.363573+00:00"}