{"created":"2023-06-20T13:23:06.243892+00:00","id":3872,"links":{},"metadata":{"_buckets":{"deposit":"7d42f7b4-0dda-4d78-84ed-9f49c9343049"},"_deposit":{"created_by":21,"id":"3872","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3872"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003872","sets":["1:283"]},"author_link":["1871","1870","345","1867","1873","1868","1830","1869","1872","1843","1875","346","1874"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"750","bibliographicPageStart":"741","bibliographicVolumeNumber":"137","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry "},{"bibliographic_title":"Journal of Biochemistry ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 正勝"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, TII0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared TII0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. TII0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the \"short\" BLUF proteins in the regulation of cyanobacterial phototaxis.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"16002996","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1093/jb/mvi089"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/jb/mvi089","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2005 The Japanese Biochemical Society"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021924X ","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKAJIMA, Koji "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHIHARA, Shizue "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUKUSHIMA, Yoshimasa "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"GENG, Xiaoxing "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KATAYAMA, Mitsunori "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIGASHI, Shoichi "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Masakatsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SATO, Shusei "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TABATA, Satoshi "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIBATA, Yutaka "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ITOH, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKEUCHI, Masahiko "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria"},{"subitem_title":"Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["109","283"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-12"},"publish_date":"2013-08-12","publish_status":"0","recid":"3872","relation_version_is_last":true,"title":["Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:29:19.090930+00:00"}