@article{oai:ir.soken.ac.jp:00003877,
 author = {渡辺, 正勝 and IWATA, Tatsuya and WATANABE, Akihide and ISEKI, Mineo and WATANABE, Masakatsu and KANDORI, Hideki},
 issue = {9},
 journal = {Journal of Physical Chemistry Letters, Journal of Physical Chemistry Letters},
 month = {May},
 note = {In the photoreaction of BLUF (sensor of blue light using FAD) domains, conserved Tyr/Gln nearby the FAD chromophore plays a crucial role in the formation of a red-shifted intermediate. The intermediate appears accompanying a hydrogen-bonding alteration of the Tyr/Gln. In order to reveal the hydrogen-bonding structure of the Tyr/Gln active center and FAD, FTIR spectroscopy was applied in the 4000-1800 cm -1 region, where X-H stretches appear. The present FTIR data show an unusually low O-H stretching vibration of tyrosine at 2800-2600 cm -1. This provides direct evidence of the light-induced switch of the hydrogen-bonding network, where the conserved Tyr (Tyr21 for AppA) donates an unusually strong hydrogen bond in the intermediate.},
 pages = {1015--1019},
 title = {Strong donation of the hydrogen bond of tyrosine during photoactivation of the BLUF domain},
 volume = {2},
 year = {2011}
}