{"created":"2023-06-20T13:23:06.720700+00:00","id":3883,"links":{},"metadata":{"_buckets":{"deposit":"0e6c4ed5-5fdd-482a-b381-25c31f548920"},"_deposit":{"created_by":21,"id":"3883","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3883"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003883","sets":["1:283"]},"author_link":["345","1865","1917","824","1843","346","1834"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1335","bibliographicPageStart":"1327","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Photochemical and Photobiological Sciences "},{"bibliographic_title":"Photochemical and Photobiological Sciences ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 正勝"}],"nameIdentifiers":[{"nameIdentifier":"345","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040124226","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"40124226","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Photoactivated adenylyl cyclase (PAC), an FAD-containing photoreceptor of Euglena gracilis, appears to be a heterotetrameric structure composed of 2 homologous subunits (PACα and PACβ), each with a pair of BLUF domains (F1 and F2). PAC promotes blue light-induced activation of adenylyl cyclase. In our previous report, we demonstrated that a recombinant version of the PACαF2 domain displays blue light-induced photocycle similar to those of prokaryotic BLUFs (Ito et al., Photochem. Photobiol. Sci., 2005, 4, 762-769). Here, we further examine the recombinant PACβF2 domain, which like PACαF2 exhibits a blue light-induced photocycle. The estimated quantum efficiency for the phototransformation of PACβF2 was 0.06-0.08, and the half-life for dark relaxation was 3-6 s while the corresponding values for the PACαF2 were 0.28-0.32 and 34-44 s. The remarkable differences between PACαF2 and PACβF2 may be related to the sensitivity of the photoactivation. In PACαF2, amino acid position 556, which is equivalent to Trp104 in the BLUF domain of the purple bacterial AppA protein, is occupied by a Leu residue, while in PACβF2 the equivalent BLUF domain site is conserved as Trp560. Amino acid substitution at this site in PACβF2-Trp560Leu markedly increased the estimated quantum efficiency (0.23) and accelerated the half-life of the dark-relaxation (2 s). These results indicate that Trp560 in PACβF2 plays a main role in suppressing the quantum efficiency.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Royal Society of Chemistry"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"20842310","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1039/c0pp00130a "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1039/c0pp00130a ","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2010 The Royal Society of Chemistry and Owner Societies"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1474905X ","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ITO, Shinji "}],"nameIdentifiers":[{"nameIdentifier":"1917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MURAKAMI, Akio "}],"nameIdentifiers":[{"nameIdentifier":"1865","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISEKI, Mineo "}],"nameIdentifiers":[{"nameIdentifier":"824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAKAHASHI, Tetsuo "}],"nameIdentifiers":[{"nameIdentifier":"1834","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HIGASHI, Shoichi "}],"nameIdentifiers":[{"nameIdentifier":"1843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"WATANABE, Masakatsu"}],"nameIdentifiers":[{"nameIdentifier":"346","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis"},{"subitem_title":"Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["109","283"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-13"},"publish_date":"2013-08-13","publish_status":"0","recid":"3883","relation_version_is_last":true,"title":["Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:29:10.333636+00:00"}