{"created":"2023-06-20T13:23:08.282065+00:00","id":3911,"links":{},"metadata":{"_buckets":{"deposit":"815e2d0a-1d8d-4c0f-b3a0-96a95dfa2f27"},"_deposit":{"created_by":21,"id":"3911","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3911"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003911","sets":["1:283"]},"author_link":["345","1970","1971","1868","836","1875","346"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"2224","bibliographicPageStart":"2214","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Plant and Cell Physiology "},{"bibliographic_title":"Plant and Cell Physiology ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 正勝"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two wild-type substrains of a motile cyanobacterium Synechocystis sp. PCC 6803 show positive phototaxis toward a light source (PCC-P) and negative phototaxis away from light (PCC-N). In this study, we found that a novel two-component system of photoresponse is involved in the phototactic regulation. Inactivation of slr1212 (pixA), which encodes a photoreceptor histidine kinase, reverted the positive phototaxis of PCC-P to negative phototaxis, and inactivation of the downstream slr1213 (nixB) and slr1214 (nixC), which encode AraC-like transcription factor-type and PatA-type response regulators, respectively, reverted the negative phototaxis of PCC-N to positive phototaxis. Opposite effects of pixA and nixBC disruption implies an unexpected signal transduction pathway in the switching of positive and negative phototaxis. The blue/green-type cyanobacteriochrome GAF domain of PixA was expressed in Synechocystis and phycocyanobilin-producing Escherichia coli. The holoprotein covalently bound a chromophore phycoviolobilin and showed reversible photoconversion between the violet-(Pv, λ peak = 396 nm) and green-absorbing (Pg, λ peak = 533 nm) forms, although the protein from E. coli partially bound a precursor phycocyanobilin. These results were discussed with regard to an idea that PixA serves as a violet light receptor for switching of positive and negative phototaxis by transcriptional and functional regulation.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"22065076","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1093/pcp/pcr155 "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/pcp/pcr155","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 The Author"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00320781 ","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NARIKAWA, Rei "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Fumiko "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHIHARA, Shizue "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIGASHI, Sho-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Masakatsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKEUCHI, Masahiko "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803"},{"subitem_title":"Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["109","283"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-22"},"publish_date":"2013-08-22","publish_status":"0","recid":"3911","relation_version_is_last":true,"title":["Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:28:55.855722+00:00"}