{"created":"2023-06-20T13:23:08.859310+00:00","id":3925,"links":{},"metadata":{"_buckets":{"deposit":"f608d2d0-3382-4f97-8c45-cc092b623c88"},"_deposit":{"created_by":21,"id":"3925","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3925"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003925","sets":["1:283"]},"author_link":["2007","345","824","2008","346","2009"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"1521","bibliographicPageStart":"1517","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Plant Physiology "},{"bibliographic_title":"Plant Physiology ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡辺, 正勝"}],"nameIdentifiers":[{"nameIdentifier":"345","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040124226","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"40124226","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Euglena gracilis, a unicellular freshwater protist exhibits different photomovement responses, such as phototaxis (oriented movement toward or away from the light source) and photophobic (abrupt turn in response to a rapid increase [step-up] or decrease [step-down] in the light fluence rate) responses. Photoactivated adenylyl cyclase (PAC) has been isolated from whole-cell preparations and identified by RNA interference (RNA) to be the photoreceptor for step-up photophobic responses but not for step-down photophobic responses (M. Iseki, S, Matsunaga, A. Murakami, K. Ohno, K. Shiga, C. Yoshida, M. Sugai, T. Takahashi, T. Hori, M. Watanabe [2002] Nature 415: 1047-1051). The present study shows that knockdown of PAC by RNAi also effectively suppresses both positive and negative phototaxis, indicating for the first time that PAC or a PAC homolog is also the photoreceptor for photoorientation of wild-type E. gracilis. Recovery from RNAi occurred earlier for step-up photophobic responses than for positive and negative phototaxis. In addition, we investigated several phototaxis mutant strains of E. gracilis with different cytological features regarding the stigma and paraxonemal body (PAB; believed to be the location for the phototaxis photoreceptor) as well as Astasia longa, a close relative of E. gracilis. All of the E. gracilis mutant strains had PAC mRNAs, whereas in A. longa, a different but similar mRNA was found and designated AlPAC. Consistently, all of these strains showed no photo taxis but performed step-up photophobic responses, which were suppressed by RNAi of the PAC mRNA. The fact that some of these strains possess a cytologically altered or no PAB demonstrates that at least in these strains, the PAC photoreceptor responsible for the step-up photophobic responses is not located in the PAB.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society of Plant Biologists"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"14630964","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1104/pp.103.034223 "}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1104/pp.103.034223","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Copyright"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plantcell.org/site/misc/ifora.xhtml","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 by the American Society of Plant Biologists"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00320889 ","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NTEFIDOU, Maria"}],"nameIdentifiers":[{"nameIdentifier":"2007","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ISEKI, Mineo "}],"nameIdentifiers":[{"nameIdentifier":"824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"WATANABE, Masakatsu"}],"nameIdentifiers":[{"nameIdentifier":"346","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"LEBERT, Michael "}],"nameIdentifiers":[{"nameIdentifier":"2008","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HäDER, Donat-Peter "}],"nameIdentifiers":[{"nameIdentifier":"2009","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-08-26"}],"displaytype":"simple","filename":"1517.full.pdf","filesize":[{"value":"163.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"1517.full","url":"https://ir.soken.ac.jp/record/3925/files/1517.full.pdf"},"version_id":"fb9e75b4-4c1a-479e-92b9-f413d558cb27"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis"},{"subitem_title":"Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["109","283"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-26"},"publish_date":"2013-08-26","publish_status":"0","recid":"3925","relation_version_is_last":true,"title":["Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:28:46.881087+00:00"}