@article{oai:ir.soken.ac.jp:00003932, author = {渡辺, 正勝 and MATSUNAGA, Shigeru and HORI, Terumitsu and TAKAHASHI, Tetsuo and MAMORU, Kubota and WATANABE, Masakatsu and OKAMOTO, Kazuhisa and MASUDA, Ketsutada and SUGAI, Michizo}, issue = {1-2}, journal = {Protoplasma, Protoplasma}, month = {}, note = {Cultures of unicellular algal flagellate Euglena gracilis grown in different conditions were subjected to action spectroscopy for step-down and step-up photophobic responses, respectively. The spectral region was extended into the UV-B/C as well as in the UV-A and visible regions with the Okazaki Large Spectrograph as the monochromatic light source. The photophobic responses of the cells were measured with an individual-cell assay method with the aid of a computerized video motion analyzer. In the UV-A and visible regions, the shapes of the action spectra were the so-called UV-A/blue type. In the newly studied UV-B/C region, new action peaks were found at 270 nm for the step-down response and at 280 nm for the step-up one. The absorption spectrum of flavin adenine dinucleotide (FAD) appeared to fit the action spectrum for the step-up response, whereas the shape of the step-down action spectrum, which has a UV-A peak (at 370 nm) higher than the blue peak (at 450 nm), appeared to he mimicked by the absorption spectrum of a mixed solution of 6-biopterin and FAD. These observations might also account for the fact that the UV-B/C peak wavelength at 270 nm of the action spectrum for the step-down response is shorter by 10 nm than the action spectrum for the step-up response at 280 nm.}, pages = {45--52}, title = {Discovery of signaling effect of UV-B/C light in the extended UV-A/blue-type action spectra for step-down and step-up photophobic responses in the unicellular flagellate alga Euglena gracilis}, volume = {201}, year = {1998} }