@article{oai:ir.soken.ac.jp:00003956,
 author = {田辺, 秀之 and UDAGAWA, Koji and KIMURA, Hajime and TANABE, Hideyuki and OHYAMA, Takashi},
 issue = {4},
 journal = {Journal of Bioscience and Bioengineering, Journal of Bioscience and Bioengineering},
 month = {Apr},
 note = {Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus.},
 pages = {431--437},
 title = {Nuclear localization of reporter genes activated by curved DNA},
 volume = {113},
 year = {2012}
}