{"created":"2023-06-20T13:23:10.806936+00:00","id":3956,"links":{},"metadata":{"_buckets":{"deposit":"984f07fc-d334-4658-884a-da7b8375387f"},"_deposit":{"created_by":21,"id":"3956","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3956"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003956","sets":["1:293"]},"author_link":["2062","368","367","2061","2063"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"437","bibliographicPageStart":"431","bibliographicVolumeNumber":"113","bibliographic_titles":[{"bibliographic_title":"Journal of Bioscience and Bioengineering"},{"bibliographic_title":"Journal of Bioscience and Bioengineering","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田辺, 秀之"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.jbiosc.2011.11.022"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbiosc.2011.11.022","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 The Society for Biotechnology, Japan."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1389-1723","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"UDAGAWA, Koji "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIMURA, Hajime "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANABE, Hideyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHYAMA, Takashi"}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nuclear localization of reporter genes activated by curved DNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nuclear localization of reporter genes activated by curved DNA"},{"subitem_title":"Nuclear localization of reporter genes activated by curved DNA","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["293"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-09-18"},"publish_date":"2013-09-18","publish_status":"0","recid":"3956","relation_version_is_last":true,"title":["Nuclear localization of reporter genes activated by curved DNA"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:28:27.774640+00:00"}