@article{oai:ir.soken.ac.jp:00003957,
 author = {WATANABE, Takaaki and TANABE, Hideyuki and HORIUCHI, Takashi},
 issue = {16},
 journal = {Nucleic Acids Research, Nucleic Acids Research},
 month = {Sep},
 note = {application/pdf, Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells.},
 title = {Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification},
 volume = {39},
 year = {2011}
}