{"created":"2023-06-20T13:23:10.871216+00:00","id":3957,"links":{},"metadata":{"_buckets":{"deposit":"e14854aa-0257-4c67-8b56-f270c914bd6c"},"_deposit":{"created_by":21,"id":"3957","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3957"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003957","sets":["1:293"]},"author_link":["2064","2065","368"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageStart":"e106","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Nucleic Acids Research"},{"bibliographic_title":"Nucleic Acids Research","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells. ","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1093/nar/gkr442"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/nar/gkr442","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Copyright"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.oxfordjournals.org/access_purchase/self-archiving_policyc.html","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2011"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0305-1048","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"WATANABE, Takaaki "}],"nameIdentifiers":[{"nameIdentifier":"2064","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TANABE, Hideyuki"}],"nameIdentifiers":[{"nameIdentifier":"368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"HORIUCHI, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"2065","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-09-18"}],"displaytype":"simple","filename":"e106.full.pdf","filesize":[{"value":"6.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"e106.full","url":"https://ir.soken.ac.jp/record/3957/files/e106.full.pdf"},"version_id":"a4dbe9ef-8299-4a24-a9a6-c7aaef56d212"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification"},{"subitem_title":"Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["112","293"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-09-18"},"publish_date":"2013-09-18","publish_status":"0","recid":"3957","relation_version_is_last":true,"title":["Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:16:27.088910+00:00"}