{"created":"2023-06-20T13:23:12.772460+00:00","id":3988,"links":{},"metadata":{"_buckets":{"deposit":"9d87e8d5-bc09-4b76-ad7e-22eb75b9c1b0"},"_deposit":{"created_by":21,"id":"3988","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3988"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00003988","sets":["1:293"]},"author_link":["2144","3119","368","367","2108"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"251","bibliographicPageStart":"245","bibliographicVolumeNumber":"307","bibliographic_titles":[{"bibliographic_title":"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis "},{"bibliographic_title":"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田辺, 秀之"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Spontaneously existing and chemically induced micronuclei were isolated from mouse blood. 50 μl of cardiac blood was diluted with PBS and centrifuged. After this, the cell pellet was subjected to hypotonic treatment, fixed with acetic acid-methanol (1:3), and the lysate was filtrated through a 2-μm polycarbonate nuclepore membrane. Isolated micronuclei were air-dried on a glass slide and subjected to fluorescence in situ hybridization (FISH) using a mouse centromeric gamma satellite probe. Approximately half of the micronuclei isolated from vehicle control mice showed centromere signal(s). In these preliminary studies, the proportion of centromere-positive micronuclei was increased by treatment with spindle poisons (colchicine and vinblastine sulfate), decreased only slightly by 1-β-D-arabinofuranosylcytosine, and was generally unaffected by mitomycin C.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0027-5107(94)90298-4"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0027-5107(94)90298-4","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1994 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0921-8262","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HAYASHI, Makoto "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MäKI-PAAKKANEN, Jorma "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANABE, Hideyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"et, al."}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe"},{"subitem_title":"Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["293"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-09-25"},"publish_date":"2013-09-25","publish_status":"0","recid":"3988","relation_version_is_last":true,"title":["Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:28:09.084901+00:00"}