{"created":"2023-06-20T13:23:18.413928+00:00","id":4099,"links":{},"metadata":{"_buckets":{"deposit":"32392e9c-670b-43a8-a597-5e6257746c16"},"_deposit":{"created_by":21,"id":"4099","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4099"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004099","sets":["2:431:25"]},"author_link":["2337","2339","2338"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小川, 裕理"}],"nameIdentifiers":[{"nameIdentifier":"2337","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"オガワ, ユリ"}],"nameIdentifiers":[{"nameIdentifier":"2338","nameIdentifierScheme":"WEKO"}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2013-03-22"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"The compound eye of Colias erate is composed of about 6,500 ommatidia, each\ncontaining nine photoreceptors (R1-9), forming a tiered rhabdom, surrounded by four\nreddish pigments. The ommatidia are divided into three types (I-III) due to the\narrangement of perirhabdormal pigment spots and expression pattern of four opsin\nmRNAs. The opsins are already identified: they are short (CeUV), two middle (CeV1,\nCeV2) and L (CeL)-absorbing types. In the thesis research, I studied the physiological\nbasis of color vision in the Eastern Pale Clouded yellow butterfly, Colias erate Esper, in\ndetail focusing on the production mechanism of the variety of spectral photoreceptors.\nFollowing General Introduction, I describe a few basic properties of the Colias eye in\nChapter 1. The basic properties are the eyeshine, which is a reflection from the tapetum at\nthe bottom of the ommatidia, and the spectral sensitivity of the entire eye. The eyeshine of\nthe dorsal region has identical reflectance spectrum in both sexes. In the ventral region of\nmales, type I ommatidia reflect 660 nm and type II and III ommatidia reflect 730 nm. In\nfemales, type II ommatidia have broadband reflection from 620 to 730 nm, while type I\nand III respectively reflect 660 nm and 730 nm as in males. Remarkable sexual difference\nof eyeshine spectrum in type II ommatidia is attributable to the female-specific orange\npigment. Regionalization was also found in the eye spectral sensitivity determined by\nelectroretinography. Dorsal region of the retina is relatively more sensitive below 420 nm\nrange, whereas the ventral region is more sensitive in the wavelength range of 420 nm to\n550 nm. This is probably because of the differential distribution of different ommatidial\ntypes.\nFour of nine photoreceptor cells contribute their rhabdomeral microvilli to the distal\ntier of the tired Colias rhabdom. In chapter 2, I analyzed the visual pigments and spectral\nsensitivities of individual photoreceptors in the distal tier in both sexes of Colias erate. A\nsubset of photoreceptor cells expresses a newly discovered middle wavelength-absorbing\nopsin, Colias erate Blue (CeB), in addition to two previously described middle\nwavelength-absorbing opsins, CeV1 and CeV2. The other photoreceptors either coexpress\nCeV1 and CeV2, or exclusively express a short wavelength-absorbing opsin, CeUV, or a\nlong wavelength-absorbing opsin, CeL. Males and females have the same visual\npigment expression patterns, but the photoreceptor spectral sensitivities are sexually\ndimorphic. The photoreceptors coexpressing three middle wavelength-absorbing opsins\nare broad-blue receptors in males, but in females they are narrow-blue receptors. Those\nwith CeV1 and CeV2 are violet receptors in females, while they are shouldered-blue\nreceptors in males. The sexual dimorphism in spectral sensitivity is caused by a\nsex-specific distribution of fluorescent pigment that functions as a spectral filter.\nIn chapter 3, I demonstrated by intracellular recording that the spectral\nsensitivities of the proximal photoreceptors (R5-8) of all ommatidia in both sexes are\nstrongly tuned by the perirhabdomal pigments. These act as long-pass filters, shifting the\npeak sensitivities into the wavelength range above 600 nm. Due to the sex-specific\npigments in type II ommatidia, the spectral sensitivities of the R5-8 photoreceptors of\nfemales peaked at 620 nm while those in males peaked at 660 nm. The measured\nspectral sensitivities could be well reproduced by an optical model assuming a long\nwavelength-absorbing visual pigment with peak absorbance at 565 nm. Whereas the\nsexual dimorphism was unequivocally demonstrated for the ventral eye region,\ndimorphism in the dorsal region was not found. Presumably the ventral region is\nstrongly related to sexual behaviors such as courtship and oviposition.\nI demonstrated in this thesis that males and females of Colias have different set\nof eight classes of spectral photoreceptor in the ventral region of the eye. On the other\nhand, I did not detect any sexual dimorphism in the dorsal retina. The eight types of\nphotoreceptor identified in the Colias ventral retina are generated by 1) duplication of\nopsin genes, 2) expression of multiple opsins in single photoreceptors and 3) the filtering\neffect of the fluorescent and perirhabdomal pigment. Together with previous studies,\nespecially on the small white, Pieris rapae, and the Japanese yellow swallowtail Papilio\nxuthus, these mechanisms underlying various spectral receptors appear to be widespread\namong butterflies.","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第1619号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"先導科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"23 生命共生体進化学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2012"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OGAWA, Yuri ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2339","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-26"}],"displaytype":"simple","filename":"甲1619_要旨.pdf","filesize":[{"value":"181.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨","url":"https://ir.soken.ac.jp/record/4099/files/甲1619_要旨.pdf"},"version_id":"2f50dddc-7f95-4148-8ec5-c70a381cb1c8"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲1619_本文.pdf","filesize":[{"value":"16.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/4099/files/甲1619_本文.pdf"},"version_id":"1edcc92b-f175-4197-94a7-273981ae7aad"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate"},{"subitem_title":"The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate","subitem_title_language":"en"}]},"item_type_id":"1","owner":"21","path":["25"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-11-27"},"publish_date":"2013-11-27","publish_status":"0","recid":"4099","relation_version_is_last":true,"title":["The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T15:14:29.703423+00:00"}