{"created":"2023-06-20T13:23:19.159118+00:00","id":4115,"links":{},"metadata":{"_buckets":{"deposit":"d289b902-516b-4b67-b5a7-2e1805d12a2d"},"_deposit":{"created_by":21,"id":"4115","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4115"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004115","sets":["1:323"]},"author_link":["2361","2364","2362","2366","2363"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1977-01-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"208","bibliographicPageStart":"200","bibliographicVolumeNumber":"490","bibliographic_titles":[{"bibliographic_title":"Biochimica et Biophysica Acta "},{"bibliographic_title":"Biochimica et Biophysica Acta ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The reversible unfolding from the native (N) state to the acid (A) state of α-lactalbumin by guanidine·HCl (0.8–2.0 M) was studied at 10–35 °C by means of difference spectra and pH-jump measurements. At each temperature, all points plotted as the logarithmic equilibrium constant log KNA of the N ⇌ A process against pH could fall on a curve independent of the denaturant concentration by shifting each point along the log KNA axis, where the shift factor ƒ did not depend on temperature. Moreover, by shifting the points at each temperature along the log () axis, a master curve, independent of both temperature and the denaturant concentration, could be obtained for the pH-dependence of log KNA. From the dependence of the logarithmic rate constants on pH, master curves independent of both temperature and the denaturant concentration could also be made for the  and the  processes, where A∗ means the activated state. The results show the two-state character of the N ⇌ A process.\n\nThe enthalpy changes and the differences in heat capacity for the  processes were determined from the accurate measurements of temperature dependence of the unfolding at pH 4.3 and 1.0 M guanidine·HCl. The results show that the disruption of hydrophobic interaction is caused mainly in the  process, while most of the changes in the pK values of the ionizing groups are caused in the  process.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0005-2795(77)90120-9"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0005-2795(77)90120-9","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1977 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NITTA, Katsutoshi "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KITA, N"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGAI, Shintaro "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature"},{"subitem_title":"Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-12-24"},"publish_date":"2013-12-24","publish_status":"0","recid":"4115","relation_version_is_last":true,"title":["Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:37.598058+00:00"}