{"created":"2023-06-20T13:23:19.340686+00:00","id":4119,"links":{},"metadata":{"_buckets":{"deposit":"a76f3cf0-ca7d-4732-b7a8-a07bd630f234"},"_deposit":{"created_by":21,"id":"4119","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4119"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004119","sets":["1:323"]},"author_link":["2363","2361","2364"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1978-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"254","bibliographicPageStart":"247","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Biophysical Chemistry"},{"bibliographic_title":"Biophysical Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The thermal unfolding of α-lactalbumin has been studied by equilibrium measurements of aromatic difference spectra, and by kinetic measurements of the Joule heating temperature-jump. The unfolding at neutral pH is a reversible two-state transition. The equilibrium transition curves are analyzed by the nonlinear least squares method, which gives correct values of thermodynamic parameters based on the data in a wide range of temperature. The results are discussed in relation to the previous studies on the unfolding by guanidine hydrochloride or by acid. The thermally unfolded state, a partially unfolded species, is shown to be thermodynamically similar to but not identical with the acid state. The folding pathway deduced from the kinetic results is essentially consistent with the folding model of α-lactalbumin proposed previously. Large decreases in entropy and in heat capacity during the reversed activation suggest the packing of the folded segments by hydrophobic interactions, while the forward activation shows a marked temperature dependence, probably caused by the disruption of specific long-range interactions.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0301-4622(78)87006-9"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0301-4622(78)87006-9","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1978 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-4622","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGAI, Shintaro "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Equilibrium and kinetics of the thermal unfolding of α-lactalbumin. The relation to its folding mechanism","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Equilibrium and kinetics of the thermal unfolding of α-lactalbumin. The relation to its folding mechanism"},{"subitem_title":"Equilibrium and kinetics of the thermal unfolding of α-lactalbumin. The relation to its folding mechanism","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-12-24"},"publish_date":"2013-12-24","publish_status":"0","recid":"4119","relation_version_is_last":true,"title":["Equilibrium and kinetics of the thermal unfolding of α-lactalbumin. The relation to its folding mechanism"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:35.179109+00:00"}