{"created":"2023-06-20T13:23:19.763985+00:00","id":4127,"links":{},"metadata":{"_buckets":{"deposit":"440e0331-679e-4560-84a2-d6d90706a096"},"_deposit":{"created_by":21,"id":"4127","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4127"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004127","sets":["1:323"]},"author_link":["2361","2364","2376"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1983-09-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"297","bibliographicPageStart":"281","bibliographicVolumeNumber":"169","bibliographic_titles":[{"bibliographic_title":"Journal of Molecular Biology"},{"bibliographic_title":"Journal of Molecular Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{"nameIdentifier":"2364","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070091444","nameIdentifierScheme":"NRID","nameIdentifierURI":" "},{"nameIdentifier":"70091444","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The locations have been found of the eight most slowly exchanging peptide protons in residues 1 to 19 of ribonuclease S. The resonance lines of these eight protons are resolved by proton magnetic resonance at 360 MHz when either S-peptide (residues 1 to 19) or peptide 1-15 is bound to S-protein (residues 21 to 124). Other peptide protons have been removed by exchange in the sample preparation ([1H]S-peptide is added to deuterated S-protein in D2O), and also by exchange-out of the less protected protons in residues 1 to 19.\n\nAt pH 5·1, 0°C, there is a 100-fold difference in rates of exchange between the eight most protected protons and the less protected protons of S-peptide. The highly protected protons are protected 104-fold compared to free S-peptide. The protected protons have been identified by 1H nuclear magnetic resonance after denaturing ribonuclease S in ≥3 m-urea-d4, D2O, pH 2·3, −4°C, followed by comparing the chemical shifts of the remaining eight protons with the known -NH spectrum of the free peptide, which has been assigned from the two-dimensional homonuclear correlated spectrum and by comparison with earlier work.\n\nThe eight highly protected NH protons are localized in one segment, residues 7 to 14. All eight protons are H-bonded: those of residues 7 to 13 are H-bonded within the 3-13 α-helix and that of residue 14 is H-bonded to the β-sheet. The NH proton of residue 16, which also is H-bonded to the β-sheet, is not one of the highly protected protons. Both the N atoms of the eight NH groups and also the O atoms of their CO acceptor groups are shielded from solvent in most cases, according to the molecular area calculations of Finney (1978).","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/S0022-2836(83)80184-3"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/S0022-2836(83)80184-3","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1983 Published by Elsevier Ltd."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-2836","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{"nameIdentifier":"2361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"BALDWIN, Robert L"}],"nameIdentifiers":[{"nameIdentifier":"2376","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S"},{"subitem_title":"Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-12-25"},"publish_date":"2013-12-25","publish_status":"0","recid":"4127","relation_version_is_last":true,"title":["Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:14:58.930004+00:00"}