{"created":"2023-06-20T13:23:20.444491+00:00","id":4144,"links":{},"metadata":{"_buckets":{"deposit":"6eb3a993-b738-4ff9-9bbf-e33041393479"},"_deposit":{"created_by":21,"id":"4144","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4144"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004144","sets":["1:323"]},"author_link":["2361","2389","2364","2392","2363","2391","2390"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-04-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"56","bibliographicPageStart":"51","bibliographicVolumeNumber":"263","bibliographic_titles":[{"bibliographic_title":"FEBS Letters  "},{"bibliographic_title":"FEBS Letters  ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The refolding kinetics of the tryptophan synthase β2 subunit have been investigated by circular dichroism (CD) and binding of a fluorescent hydrophobic probe (ANS), using the stopped-flow technique. The kinetics of regain of the native far UV CD signal show that, upon refolding of urea denatured β2, more than half of the protein secondary structure is formed within the dead time of the CD stopped-flow apparatus (0.013 s). On the other hand, upon refolding of guanidine unfolded β2 the fluorescence of ANS passes through a maximum after about 1 s and then ‘slowly’ decreases. These results show the accumulation, in the 1–10 s time range, of an early transient folding intermediate which has a pronounced secondary structure and a high affinity for ANS. In this time range, the near UV CD remains very low. This transient intermediate thus appears to have all the characteristics of the ‘molten globule’ state [(1987) FEBS Lett. 224, 9-13]. Moreover, by comparing the intrinsic time of the disappearance of this transient intermediate (t 35 s) with the time of formation of the previously characterized [(1988) Biochemistry 27, 7633-7640] early imuno-reactive intermediate recognized by a monoclonal antibody (t 12 s), it is shown that this native-like epitope forms within the ‘molten globule’, before the tight packing of the protein side chains.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"1691989","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0014-5793(90)80703-L"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0014-5793(90)80703-L","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Copyright"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/journal-authors/author-rights-and-responsibilities","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1990 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-5793 ","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"GOLDBERG, Michel E"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SEMISOTNOV, Gennady V"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FRIGUET, Bertrand "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"PTITSYN, Oleg B"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGAI, Shintaro "}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-15"}],"displaytype":"simple","filename":"1-s2.0-001457939080703L-main.pdf","filesize":[{"value":"854.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"1-s2.0-001457939080703L-main","url":"https://ir.soken.ac.jp/record/4144/files/1-s2.0-001457939080703L-main.pdf"},"version_id":"db40c7a0-22ea-4fea-a1f2-1a56ba10b367"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’"},{"subitem_title":"An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-15"},"publish_date":"2014-01-15","publish_status":"0","recid":"4144","relation_version_is_last":true,"title":["An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:23.184914+00:00"}