{"created":"2023-06-20T13:23:20.529148+00:00","id":4146,"links":{},"metadata":{"_buckets":{"deposit":"82c33d52-cc2c-48a6-838d-6c6864d9c926"},"_deposit":{"created_by":21,"id":"4146","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4146"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004146","sets":["1:323"]},"author_link":["2361","2393","2364","2397","2363","2398","2399"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"117","bibliographicPageStart":"111","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"Biophysical Chemistry"},{"bibliographic_title":"Biophysical Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Affinity chromatography, fluorescence and circular dichroism spectroscopy methods have been used to study the interaction of melittin, a 26-residue peptide from bee venom, with Ca2+-binding α-lactalbumin from human milk. It has been revealed that melittin binds to the apo- and acidic states of α-lactalbumin while the presence of Ca2+ makes the interaction essentially weaker. The association constant for the complex of melittin with apo-α-lactalbumin determined from spectropolarimetric melittin-titration data is 2×107 M−1. The complexation of α-lactalbumin with melittin decreases its affinity to Ca2+ by three orders of magnitude. The interaction of apo-α-lactalbumin with melittin causes some changes in the environment of its aromatic amino acid residues and drastically alters the conformation of melittin, increasing its α-helical content but leaving its single tryptophan residue accessible to water. In the case of the acidic state of α-lactalbumin the interaction does not induce an increase in α-helical content of melittin.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2059662","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0301-4622(91)85012-F"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0301-4622(91)85012-F","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1991 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-4622","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"PERMYAKOV, Eugene A"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"GRISHCHENKO, Valery M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KALINICHENKO, Lina P"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ORLOV, Nikolay Y"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGAI, Shintaro "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Calcium-regulated interactions of human α-lactalbumin with bee venom melittin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Calcium-regulated interactions of human α-lactalbumin with bee venom melittin"},{"subitem_title":"Calcium-regulated interactions of human α-lactalbumin with bee venom melittin","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-15"},"publish_date":"2014-01-15","publish_status":"0","recid":"4146","relation_version_is_last":true,"title":["Calcium-regulated interactions of human α-lactalbumin with bee venom melittin"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:20.625512+00:00"}