{"created":"2023-06-20T13:23:20.571277+00:00","id":4147,"links":{},"metadata":{"_buckets":{"deposit":"59245496-5f03-4e1e-aa12-a32a6b4db630"},"_deposit":{"created_by":21,"id":"4147","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4147"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004147","sets":["1:323"]},"author_link":["2395","2361","2364","2394","2363","2396"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-09-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"138","bibliographicPageStart":"135","bibliographicVolumeNumber":"290","bibliographic_titles":[{"bibliographic_title":"FEBS Letters  "},{"bibliographic_title":"FEBS Letters  ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Kinetics of unfolding and refolding of a staphylococcal nuclease mutant, in which Pro117 is replaced by glycine, have been investigated by stopped-flow circular dichroism, and the results are compared with those for the wild-type protein. In contrast to the biphasic unfolding of the wild-type nuclease, the unfolding of the mutant is represented by a single-phase reaction, indicating that the biphasic unfolding for the wild-type protein is caused by cis-trans isomerization about the prolyl peptide bond in the native state. The proline mutation also simplifies the kinetic refolding. Importance of the results in elucidating the folding mechanism is discussed.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"1915864","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/0014-5793(91)81243-2"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/0014-5793(91)81243-2","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Copyright"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/journal-authors/author-rights-and-responsibilities","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 1991 Published by Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-5793 ","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKAYAMA, Naoki "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAMOTO, Kaori "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ISHIHARA, Tsuyoshi "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUGAI, Shintaro "}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-01-15"}],"displaytype":"simple","filename":"1-s2.0-0014579391812432-main.pdf","filesize":[{"value":"490.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"1-s2.0-0014579391812432-main","url":"https://ir.soken.ac.jp/record/4147/files/1-s2.0-0014579391812432-main.pdf"},"version_id":"e4535a45-369a-4316-b1ca-6b585c76fddd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics"},{"subitem_title":"The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-15"},"publish_date":"2014-01-15","publish_status":"0","recid":"4147","relation_version_is_last":true,"title":["The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:19.738197+00:00"}