{"created":"2023-06-20T13:23:20.783034+00:00","id":4152,"links":{},"metadata":{"_buckets":{"deposit":"ee50d2f1-c1da-499b-9150-4bc619bc365f"},"_deposit":{"created_by":21,"id":"4152","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4152"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004152","sets":["1:323"]},"author_link":["2361","2364","2408","2409","2407"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"446","bibliographicPageStart":"439","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"Nature Structural Biology"},{"bibliographic_title":"Nature Structural Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigate here the interaction between GroEL and two kinds of non-native -lactalbumin. -Lactalbumin is a Ca2+-binding protein which assumes a molten globule state in the absence of Ca2+ (apo--lactalbumin) at neutral pH. Our results, obtained by molecular-sieve chromatography and hydrogen-exchange measurements, show that apo--lactalbumin in this molten globule state is not bound to GroEL either in the absence or in the presence of KCl. On the other hand, we show by molecular-sieve chromatography that -lactalbumin, in which the four disulphide bonds are fully reduced, is bound to GroEL when 50 mM KCl is present. The results demonstrate that the protein state recognized by GroEL is more unfolded and expanded than the typical molten globule state of -lactalbumin.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/nsb0794-439 "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/nsb0794-439","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©1994 Nature Publishing Group"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1545-9993","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKAZAKI, Akira "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKURA, Teikichi "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NIKAIDO, Kiyokazu "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state"},{"subitem_title":"The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-17"},"publish_date":"2014-01-17","publish_status":"0","recid":"4152","relation_version_is_last":true,"title":["The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:27:13.656447+00:00"}