@article{oai:ir.soken.ac.jp:00004226,
 author = {渡辺, 正勝 and HASUNUMA, Kohji and FURUKAWA, Kazuhiko and FUNADERA, Kanako and KUBOTA, Mamoru and WATANABE, Masakatsu},
 issue = {4},
 journal = {Photochemistry and Photobiology, Photochemistry and Photobiology},
 month = {Oct},
 note = {Binding of GTP-binding proteins with [35S]GTP7S in the extract containing membrane components of Lemna paucicostata 441 was inhibited by red or far red light by 20 to 25%, but blue light showed no or little effect. The plant used for the preparation of the extract was subjected to single darkness for 8 h, as both red and far red light inhibit flowering. The extract treated with 1% Lubrol was fractionated by gel filtration. Four species of GTP-binding proteins, GL1, GL2, GL3 and GL4 were detected with Km values 3, 7, 80 and 4 nM, respectively. GL1, GL2 and GL3 were ADP-ribosylated by pertussis toxin. The extract activated by [35S]GTP-γS in darkness, under red light or under far red light was treated with 1% Lubrol and subsequent gel filtration of the extracts made it possible to detect GTP-binding protein with a small molecular weight only in an extract labeled in darkness. The reduction in the molecular weight of GTP-binding protein from the larger molecule associated with the binding of [35S]GTPγS was confirmed by rechromatography of the larger molecule activated by [35S]GTPγS in darkness. The binding of GL2 and/or GL3 with [35S]GTPγS was suggested to be inhibited by red or far red light.},
 pages = {531--535},
 title = {Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata.},
 volume = {46},
 year = {1987}
}