{"created":"2023-06-20T13:23:27.368895+00:00","id":4310,"links":{},"metadata":{"_buckets":{"deposit":"9d902d4f-fb15-4ef4-a5e1-e37df3dbadb2"},"_deposit":{"created_by":21,"id":"4310","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4310"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004310","sets":["1:323"]},"author_link":["2516","2361","2364"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2-3","bibliographicPageEnd":"212","bibliographicPageStart":"203","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"Spectroscopy: An International Journal"},{"bibliographic_title":"Spectroscopy: An International Journal","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The folding mechanism of proline-free staphylococcal nuclease (SNase (pro-)) (P11A, P31A, P42A, P47T, P56A, P117G) was investigated using the double-jump stopped-flow method (interrupted refolding). This method has enabled us to specifically monitor the amount of the native molecules during the refolding. The results indicate that the middle and slow phases observed in the refolding kinetics represent the formation of the native state (IM → N, IS → N) and that the folding mechanism of SNase (pro-) is not represented by a single sequential pathway, but at least two parallel pathways are required for interpreting the results.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"IOS Press"}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"IOS Press "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://content.iospress.com/articles/spectroscopy/spe097","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2003 IOS Press "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0712-4813","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KAMAGATA, Kiyoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method"},{"subitem_title":"Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-03-18"},"publish_date":"2014-03-18","publish_status":"0","recid":"4310","relation_version_is_last":true,"title":["Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:25:23.231539+00:00"}