{"created":"2023-06-20T13:23:27.588866+00:00","id":4315,"links":{},"metadata":{"_buckets":{"deposit":"a1c93d23-6d70-4a94-a4be-2eb90090a346"},"_deposit":{"created_by":21,"id":"4315","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4315"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004315","sets":["1:323"]},"author_link":["2364","2361","3119","2519"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"2618","bibliographicPageStart":"2606","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"Biophysical Journal"},{"bibliographic_title":"Biophysical Journal","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/S0006-3495(03)74683-9"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/S0006-3495(03)74683-9","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Copyright"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2003 The Biophysical Society."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-3495","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ALI, Said A. "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"et, al."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-03-19"}],"displaytype":"simple","filename":"1-s2.0-S0006349503746839-main.pdf","filesize":[{"value":"316.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"1-s2.0-S0006349503746839-main","url":"https://ir.soken.ac.jp/record/4315/files/1-s2.0-S0006349503746839-main.pdf"},"version_id":"5111e3c7-0922-4811-a8d5-2b633deb6eca"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4"},{"subitem_title":"Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-03-19"},"publish_date":"2014-03-19","publish_status":"0","recid":"4315","relation_version_is_last":true,"title":["Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:25:06.480450+00:00"}