{"created":"2023-06-20T13:23:27.945295+00:00","id":4324,"links":{},"metadata":{"_buckets":{"deposit":"2997546d-efea-4b39-bf78-85ea7fe214ff"},"_deposit":{"created_by":21,"id":"4324","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4324"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004324","sets":["1:323"]},"author_link":["2361","2525","2364","3119"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-11-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"172","bibliographicPageStart":"164","bibliographicVolumeNumber":"354","bibliographic_titles":[{"bibliographic_title":"Journal of Molecular Biology "},{"bibliographic_title":"Journal of Molecular Biology ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑島, 邦博"}],"nameIdentifiers":[{},{},{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The recombinant form of goat α-lactalbumin has a significantly faster unfolding rate compared to the authentic form, although the two molecules differ only in an extra methionine at the N terminus of the recombinant. The mechanism of the destabilization caused by this residue was investigated through the combined use of kinetic experiments and molecular dynamics simulations. Unfolding simulations for the authentic and recombinant forms at 398 K (ten trajectories of 5 ns for each form, 100 ns total) precisely reproduced the experimentally observed differences in unfolding behavior. In addition, experiments reproduced the destabilization of a mutant protein, T38A, faithfully as predicted by the simulations. This bidirectional verification between experiments and simulations enabled the atomically detailed description of the role of the extra methionine residue in the unfolding process.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier "}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.jmb.2005.09.061"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jmb.2005.09.061","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2005 Elsevier Ltd. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-2836 ","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OROGUCHI, Tomotaka "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUWAJIMA, Kunihiro "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"et, al."}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations"},{"subitem_title":"Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["323"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-03-24"},"publish_date":"2014-03-24","publish_status":"0","recid":"4324","relation_version_is_last":true,"title":["Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:25:00.203789+00:00"}