@article{oai:ir.soken.ac.jp:00004612,
 author = {宅野, 将平 and OIKAWA, Eriko and TAKUNO, Shohei and et, al.},
 issue = {1},
 journal = {Molecular Breeding, Molecular Breeding},
 month = {Jun},
 note = {In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds.},
 pages = {1--12},
 title = {Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis},
 volume = {28},
 year = {2011}
}