{"created":"2023-06-20T13:23:36.248693+00:00","id":4612,"links":{},"metadata":{"_buckets":{"deposit":"1e225cf9-623e-4e5a-8648-bc7e99abeb76"},"_deposit":{"created_by":21,"id":"4612","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"4612"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00004612","sets":["1:340"]},"author_link":["768","3119","2962","2937"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"Molecular Breeding "},{"bibliographic_title":"Molecular Breeding ","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宅野, 将平"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"SpringerLink "}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/s11032-010-9455-8"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s11032-010-9455-8","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"SpringerLink(The original publication is available at www.springerlink.com) "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1380-3743","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OIKAWA, Eriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKUNO, Shohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"et, al."}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis"},{"subitem_title":"Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"21","path":["339","340"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-05"},"publish_date":"2014-06-05","publish_status":"0","recid":"4612","relation_version_is_last":true,"title":["Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis"],"weko_creator_id":"21","weko_shared_id":21},"updated":"2023-06-20T14:23:53.624883+00:00"}