@article{oai:ir.soken.ac.jp:00005560,
 author = {LAU, Quintin and IGAWA, Takeshi and KOMAKI, Shohei and SATTA, Yoko and LAU, Quintin and IGAWA, Takeshi and KOMAKI, Shohei and SATTA, Yoko},
 issue = {10},
 journal = {Immunogenetics, Immunogenetics},
 month = {Nov},
 note = {The major histocompatibility complex (MHC) is a key component of adaptive immunity in all jawed vertebrates, and understanding the evolutionary mechanisms that have shaped these genes in amphibians, one of the earliest terrestrial tetrapods, is important. We characterised MHC class I variation in three common Japanese Rana species (Rana japonica, Rana ornativentris and Rana tagoi tagoi) and identified a total of 60 variants from 21 individuals. We also found evolutionary signatures of gene duplication, recombination and balancing selection (including trans-species polymorphism), all of which drive increased MHC diversity. A unique feature of MHC class I from these three Ranidae species includes low synonymous differences per site (d S) within species, which we attribute to a more recent diversification of these sequences or recent gene duplication. The resulting higher d N/d S ratio relative to other anurans studied could be related to stronger selection pressure at peptide binding sites. This is one of the first studies to investigate MHC in Japanese amphibians and permits further exploration of the polygenetic factors associated with resistance to infectious diseases.},
 pages = {797--806},
 title = {Characterisation of major histocompatibility complex class I genes in Japanese Ranidae frogs.},
 volume = {68},
 year = {2016}
}