{"created":"2023-06-20T13:20:54.949284+00:00","id":986,"links":{},"metadata":{"_buckets":{"deposit":"c6520041-3bfe-4a09-a2c0-c421a868464f"},"_deposit":{"created_by":1,"id":"986","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"986"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00000986","sets":["2:430:20"]},"author_link":["10100","10099","10098"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山谷, 仁志"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ヤマタニ, ヒトシ"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2003-03-24"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"  In the mammalian olfactory system, each olfactory neuron expresses only one of about 1,000 different odorant receptor genes.  In the olfactory epithelium, the olfactory neurons expressing the same receptor are scattered, but their axons converge into a few topographically fixed glomeruli, the specialized synaptic structures in the olfactory bulb (OB).  Consequently, a stereotyped spatial map is constructed on the surface of the OB, in which about 1,800 glomeruli are orderly arranged in mice.  The physiological and anatomical studies show that the glomeruli receiving similar odorant molecules are grouped together and form a special domain on the OB.<br />  The information converged in the glomeruli is transmitted to the second-order neurons, mitral and tufted cells in the OB.  These neurons project axons caudally and construct the lateral olfactory tract (LOF) on the surface of the ventrolateral telencephalon.  The LOT axons eventually sprout collateral branches invading the olfactory cortex and form connections with the third-order neurons.  The olfactory cortex consists of morphologically distinct areas such as the anterior olfactory nucleus, the piriform cortex (PC), the olfactory tubercle (OT), the entorhinal cortex and the amygdala.  Each second-order neuron projects to the multiple target areas with massive sprouting of collateral branches.<br />  In contrast to the clear rule of the peripheral olfactory projection, the principal of central olfactory projection is obscure.  Although a few studies suggest specific targeting of the projections from second-order neurons in the olfactory cortex, there is not a point-to-point topographic relationship between the spatial map on the OB to any of the olfactory cortical areas, as other sensory systems.  For example, the retrograde axonal labeling shows that a single cortical region receives inputs from the mitral/tufted cells scattering over the OB.  The anterograde axonal tracing also shows that the spatial arrangement in the OB is not reflected in the olfactory cortical areas.  Furthermore, there is the evidence that the spatial representation of the OB is already lost in the LOT, in which mitral/tufted cell axons are intermingled randomly regardless of the position of their cell bodies.<br />  In the present study, I demonstrated that c-kit receptor tyrosine kinase is expressed on a fasciculated subset of axons in the LOT at each developmental stage.  Their c-kit-expressing cell bodies were distributed in the intermediate zone flanked between the ventricular zone and the mitral cell layer in the OB.  BrdU-labeling experiment showed that the newly-differentiated mitral/tufted cells radially migrating in the intermediate zone transiently expressed c-kit so that, the expression of c-kit is always fixed in the intermediate zone.  These results indicate that mitral/tufted cell axons projecting at the same developmental stage are grouped together and constitute a special assembly within the LOT bundle, regardless of the position of their cell bodies in the OB.  The newly elongating c-kit-positive axons usually occupied the ventral surface area in the LOT.  Therefore, there seems to be a developmental gradient in the organization of LOT axons from the dorsal depth to ventral surface.  These results together with the previous axonal tracing studies suggest, that arrangement of LOT axons is not, based on the topographical position of the cell bodies but the developmental status of the axons.<br />  The OT is one of the olfactory cortical areas that receive the late innervation by OB axons.  The OT receives a heavier projection from tufted cells the late-born projection neurons in the OB.  In later developmental stages, many c-kit-positive axons were observed to project into the OT selectively and directly.  This observation further supports that the OT receives the selective projection from the late-born projection neurons in the OB.<br />  In the LOT, c-kit-positive axons were always fasciculated and segregated from c-kit-negative axons.  When OB explants were cultured in the dish, c-kit-positive neturites did not fasciculate but randomly mixed with c-kit-negative neurites.  Even in the organotypic co-culture, c-kit-positive axons did not choose the superficial part but randomly elongated within the LOT bundle, intermingling with c-kit-negative axons.  Thus, the selective fasciculation of c-kit-positive axons in the LOT was not, reproduced in culture and might require developmentally ordered projection of LOT axons.<br />  The c-kit is encoded by the Wlocus in mice and the ligand for c-kit is stem cell factor(SCF), which encoded by the Sllocus in mice.  The interaction between c-kit and SCF is considered to be essential for the development of melanocytes, erythrocytes, mast cells and germ cells, because mutations in either the W or Sl locus result in serious defects in differentiation of these cells.  I examined whether c-kit or SCF was involved in the projection of LOT axons.  However, I did not detect any abnormality in the LOT projection of W/W or Sl/Sl d mutant mice, although it is still possible that more detailed analyses reveal some function of this signaling.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第686号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"18 遺伝学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2002"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMATANI, Hitoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲686_要旨.pdf","filesize":[{"value":"292.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/986/files/甲686_要旨.pdf"},"version_id":"144d2cae-fc89-4bbd-9fa0-f083423ee4b6"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲686_本文.pdf","filesize":[{"value":"897.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/986/files/甲686_本文.pdf"},"version_id":"435254ab-5ca3-4f74-bb25-cb641fa23749"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Transient expression of c-kit receptor in the immature projection neurons of the olfactory bulb","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transient expression of c-kit receptor in the immature projection neurons of the olfactory bulb"},{"subitem_title":"Transient expression of c-kit receptor in the immature projection neurons of the olfactory bulb","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"986","relation_version_is_last":true,"title":["Transient expression of c-kit receptor in the immature projection neurons of the olfactory bulb"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:40:41.117924+00:00"}