@misc{oai:ir.soken.ac.jp:00000989,
 author = {小池, 牧子 and コイケ, マキコ and KOIKE, Makiko},
 month = {2016-02-17, 2016-02-17},
 note = {The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC).  Most transport events through the NPC are mediated by transport receptor molecules.  The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal.  Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus.  Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus.  The export of β-catenin also occurs in a Ran-independent manner.  Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components.  In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells.  I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity.  Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export.  Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical.  Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC., application/pdf, 総研大甲第723号},
 title = {Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.},
 year = {}
}